LAG-3 (mouse):Fc (mouse) (rec.)
| Code | Size | Price |
|---|
| AG-40B-0039-C050 | 50 ug | £317.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Hamster
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Shipping:
Blue Ice
Storage:
-20°C
Documents
Further Information
Alternate Names/Synonyms:
Lymphocyte Activation Gene-3; FDC Protein; CD223
Biological Activity:
Induces maturation of mouse dendritic cells in vitro. Induces CD4 and CD8 antigen-specific T cells responses when coinjected s.c. in mice, together with the antigen.
Concentration:
0.5 mg/ml
EClass:
32160000
Endotoxin:
<0.01EU/µg purified protein (LAL test; Lonza).
Form (Short):
liquid
Formulation:
Liquid. In PBS.
Handling Advice:
After opening, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.PBS containing at least 0.1% BSA should be used for further dilutions.
Long Description:
Protein. The sequence coding for the 4 extracellular Ig-like domains of mouse LAG-3 (D1-D4) is fused to the Fc portion of mouse IgG2a (hinge-CH2-CH3). Source: CHO cells. Endotoxin content: <0.01EU/µg purified protein (LAL test; Lonza). Liquid. In PBS. Binds to human and mouse MHC class II. Purity: >95% (SDS-PAGE). Lymphocyte activation gene 3 (LAG-3; CD223) plays an important role in negatively regulating T cell proliferation, function and homeostasis. It is required for maximal natural and induced regulatory T cell (Treg) function. LAG-3 is closely related to the T cell co-receptor CD4 and binds to MHC class II molecules but with a significantly higher affinity than CD4.
Molecular Weight:
~180kDa (SPS-PAGE, nonreducing), ~80kDa (SPS-PAGE, reducing)
NCBI, Uniprot Number:
Q61790
Other data:
Application: Flow Cytometry: Detection of MHC class II molecules in combination with fluorescently labeled antibodies to IgG2a. Uni-Prot link Q61790: LAG-3 (mouse) http://www.uniprot.org/uniprot/Q61790
Package Type:
Plastic Vial
Product Description:
Lymphocyte activation gene 3 (LAG-3; CD223) plays an important role in negatively regulating T cell proliferation, function and homeostasis. It is required for maximal natural and induced regulatory T cell (Treg) function. LAG-3 is closely related to the T cell co-receptor CD4 and binds to MHC class II molecules but with a significantly higher affinity than CD4.
Purity:
>95% (SDS-PAGE)
Sequence:
The sequence coding for the 4 extracellular Ig-like domains of mouse LAG-3 (D1-D4) is fused to the Fc portion of mouse IgG2a (hinge-CH2-CH3).
Source / Host:
CHO cells
Specificity:
Binds to human and mouse MHC class II.
TAGs:
Fc
Transportation:
Non-hazardous
UNSPSC Category:
Other Proteins
UNSPSC Number:
12352202
Use & Stability:
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
References
LAG-3 enables DNA vaccination to persistently prevent mammary carcinogenesis in HER-2/neu transgenic BALB/c mice: P. Cappello, et al.; Cancer Res. 63, 2518 (2003)