Transficient™ and siRfficient™: Superior Transfection Activity against other Commercial Reagents in Numerous Adherent Cell Types

Transficient™ and siRfficient™ from MBL International are transfection reagents that provide superior transfection efficiency for primary cells and a wide variety of cell lines, including those “hard-to-transfect” cell lines. They are a cost effective and highly efficient tool for your downstream applications using a simple transfection protocol.

Transficient™ : DNA Transfection Reagent

This product has been developed and optimised for delivery of nucleic acids, such as plasmid DNA and mRNA into a wide variety of cell types by creating highly stable nanoparticles that facilitates cellular uptake, endosomal escape and localization to the nucleus.

Key benefits:

• High transfection efficiency into primary cells and a wide variety of cell lines
• Works with or without serum
• Easy to use
• Robust
• Very low toxicity

Five different cell lines were transfected using the Luciferase gene and the Transficient™ reagent. Expression levels were compared to competitor transfection reagents.

Products:

Transficient™ DNA Transfection Reagent samples available, please email office@caltagmedsystems.co.uk to request.

siRfficient™: siRNA Transfection Reagent

This product has been developed and optimised for gene silencing experiments. It provides the highest efficiencies for the delivery of siRNA, microRNA or antisense RNA into the cytoplasm of a wide variety of cell types, including Neuro 2a, Jurkat, K562 and LLC-1cells.

Key benefits:

• High transfection efficiency into primary cells and a wide variety of cell lines
• Works with or without serum
• Easy to use
• Robust
• Very low toxicity

Left: β-actin levels were knocked down in Jurkat cells using the siRfficient™ reagent. Compared to Company A and Company B, siRfficient™ showed a 90% knockdown level. Right: VEGF protein levels were silenced using the siRfficient™ reagent, with and without serum in a Neuro 2a cell line. The results were compared Competitor silencing reagents.

Products:

siRNA Transfection Reagent samples available, please email office@caltagmedsystems.co.uk to request.