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Diaclone

Welcome to Diaclone – distributed exclusively in the UK and Ireland by Caltag Medsystems

With over 30 years' of experience and expertise, Diaclone SAS is respected as a centre of excellence in Monoclonal Antibody and Immunoassay development. Their products & services are utilised in key research, clinical and discover applications by many research, pharmaceutical and manufacturing organisations worldwide.

Diaclone is committed to the highest level of quality in the design, development and manufacture of their products with a quality management system certified to ISO9001:2008. Through their extensive knowledge in antibody and immunoassay development, they pride themselves on the quality and responsiveness of their technical support. Diaclone also can offer the flexibility and adaptability to deliver custom development and bulk product supply.

Diaclone offers a large portfolio of monoclonal antibodies, ELISA, ELISpot & Multiplex assays in the areas of T cell Immunology, CD antigens, Cytokines, cytokine/chemokine receptors, adhesion molecules and apoptosis related antigens. Read more about their portfolio below:

ELISA

Diaclone ELISA

Diaclone ELISAs are highly sensitive assays designed for the in-vitro quantitative and qualitative determination of cytokines, CD antigens, apoptosis markers and other analytes from human serum, plasma and cell culture supernatant. These techniques are amongst the most sensitive available for analyte identification and quantification and benefit from a rapid whole-assay time and the provision of robustly validated standards, controls and antibodies. Diaclone ELISA are available for the detection of human, murine and rat proteins.

  • High Performance
  • Highly sensitive assay
  • Fast procedure
  • Flexibility of multiple formats

ELISA reagents are available in a number of formats; from whole pre-coated kits (for easy analysis and increased throughput) to matched antibody pairs (a great tool for developing research).

ELISA Kits: Pre-Coated Plates, Biotinylated Detection Antibody, standard(s), Control(s) (where available), Buffers, Streptavidin-HRP and TMB, Stop reagent and plate sealers.

ELISA Sets: Capture Antibody, Biotinylated Detection Antibody, standard(s), Streptavidin-HRP and TMB.

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Monoclonal Antibodies

Diaclone Monoclonal Antibodies

As the original manufacturer of the highly respected 'B-clones', Diaclone SAS provides highly specific, high quality mouse monoclonal antibodies for the detection of a broad range of Human proteins, including: human CD antigens, cytokines, cytokine/chemokine receptors, adhesion molecules and apoptosis related antigens.

These mAbs are available in a variety of formats including azide free, purified and conjugated (FITC, PE, Biotin) for applications in Flow Cytometry, Immunoassay, Immunohistochemistry, Western Blot, Immunoprecipitation, Biologically active antibodies for use in Biological Assays.

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View Diaclone Isotype Controls

ELISpot

Diaclone ELISpot

Diaclone ELISpots (Enzyme-Linked Immunospot Assays) are highly specific immunoassays for the analysis of cytokine and other soluble molecule production and secretion from T-cells at a single cell level in conditions closely comparable to the in-vivo environment with minimal cell manipulation.

Utilising sandwich immuno-enzyme technology, Diaclone ELISpot and Dual ELISpot assays can detect both secreted cytokines and measure the frequency of single cells that simultaneously produce multiple cytokines or other effector molecules.

ELISpot techniques are amongst the most-sensitive methods available (up to 400x more sensitive than conventional ELISA) for cytokine research and immune monitoring. The range of kits from Diacone extends across different species with high specificity and affinity for a number of different cytokines and soluble molecules.

Mono and Dual cytokine analysis is available using both enzymatic and fluorescent detection systems.

ELISpot reagents are available in a number of formats from whole pre-coated kits (for easy analysis and increased throughput) to matched Antibody Pairs (a great tool for developing research).

ELISpot Kits: Pre-coated PVDF plate(s), Detection antibody, Alkaline Phosphatase conjugate, BSA and BCIP/NBT ready-to-use substrate buffer.

ELISpot Sets: Capture and Detection antibody, Alkaline Phosphatase conjugate, BSA and BCIP/NBT ready-to-use substrate buffer.

ELISpot Pair: Capture and Detection Antibodies.

Dual ELISpot Sets: Capture and Detection antibodies for two analytes, Alkaline phosphatase conjugate, blocking reagent, Ready-to-use BCIP/NBT substrate buffer, Peroxydase conjugate, BSA, Ready-to-use AEC substrate buffer.

Dual Fluorospot Sets: Capture antibody for two analytes, FITC-conjugated Detection antibody for cytokine 1, anti-FITC antibody green fluorescence conjugate, biotinylated detection antibody for cytokine 2, streptavidin-phycoerythrin conjugate, BSA.

View Diaclone ELISpot products

DIAplex

Diaclone DIAplex

Diaclone DIAplex is a sensitive multiplex fluorescent bead-based immunoassay for the simultaneous quantification of multiple analytes from a single sample by flow cytometry.

Utilising bead populations with distinct fluorescence intensities and unique antibody specificities, Diaclone DIAplex can accurately measure multiple analytes in a single sample with a significantly reduced assay time and sample volume requirement when compared to traditional ELISA techniques.

Versatility & Simplicity

  • Test multiple sample types including serum, plasma and cell culture supernatants
  • Compatible with most commercially available flow cytometers
  • The simple to use Diaclone DIAplex Pro software enables calculation of analyte concentration from a standard curve and provision of detailed analysis reports within a matter of minutes post flow cytometry measurement
  • Robust validation for uncompromised reproducibility and sensitivity
  • Small sample volume required due to multiple analyte detection in one sample maximizing the number of analytes that can be tested in a single sample
  • A single set of diluted standards is used to generate a standard curve for each analyte
  • Takes less time than a conventional ELISA and provides data that would normally require multiple ELISA in a smaller sample volume

DIAplex Complete Sets: provides multiples assay sets which are preconfigured for the detection of a panel of analytes for investigation of a specific scientific area. An individual experiment can provide measurements of several analytes from a single sample. Available as 96 test per Analyte.

DIAplex Design: combines the benefits of multiplexing with the ability to design your own analyte detection panel. The assays have been designed for use individually (simplex) or combined to create any size flex system. A DIAplex accessory kit must be ordered with each combination. Available as 96 tests per analyte.

View Diaclone DIAplex products

If you would like further information about any of Diaclone's products, please contact us at techsupport@caltagmedsystems.co.uk or by telephone: +44 (0)1280 827460.