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3Helix has developed a series of proprietary collagen hybridizing peptides that directly target the damaged collagen molecule. Collagen is the major component of nearly every human tissue, with an essential role in supporting cell growth and tissue formation. Damage to collagen is a strong indication of connective tissue injury, as well as a variety of diseases involving inflammation and abnormal tissue remodelling, such as cancer, myocardial infarction, arthritis, osteoporosis, and fibrosis.

In addition to providing Collagen Hybridising Peptide (CHP) as a research tool for laboratory use in academia and industry, 3Helix is advancing its compounds to enable new pathways to achieving diagnostic and therapeutic objectives.

Products

The collagen hybridizing peptide (CHP) is a novel and unique peptide that specifically binds unfolded collagen chains. By sharing the Gly-X-Y repeating sequence of natural collagen, CHP has a strong capability to hybridize with denatured collagen chains by reforming the triple helical structure, in a fashion similar to DNA fragments annealing to complementary DNA strands. CHP is extremely specific: it has a negligible affinity for intact collagen molecules due to its lack of binding sites, and it is inert towards non-specific binding because of its neutral and hydrophilic nature.

Their new product line, the sulfo-Cy7.5-CHP conjugates, are the perfect tool for in vivo detection of collagen degradation. This new sequence allows the peptide to be directly injected into animals without having to pre-activate them with a heating step. Therefore, the in vivo CHPs will always be in their active form and will not self-hybridize once injected. The conjugation of the peptide to the sulfonated-cyanine 7.5 dye allows near-infrared fluorescence detection, which offers better tissue penetration while minimizing tissue autofluorescence.

Name Collagen Hybridising Peptide, 5-FAM Conjugate Collagen Hybridising Peptide, Cy3 Conjugate Collagen Hybridising Peptide, Biotin Conjugate In vivo Collagen Hybridizing Peptides, sulfo-Cy7.5 Conjugates
Product Codes 3H-FLU300
3H-FLU60
3H-RED300
3H-RED60
3H-BIO300
3H-BIO60
3H-T-sCy7.5
3H-C-sCy7.5
3H-K-sCy7.5
Size 300 μg / 60 μg 300 μg / 60 μg 300 μg / 60 μg 8 nmoles (3 doses) This is for a single vial of either the targeted or control peptides.
Specialty Straightforward fluorescence detection in green Straightforward fluorescence detection in red Flexible avidin / streptavidin detection options based on needs, allowing non-green fluorescence and HRP methods to avoid background and enhance signal Single sulfonated-cyanine 7.5 dye per peptide
Molecular weight 2952.01 g/mol 3191.44 g/mol 2762.01 g/mol 3703.1 g/mol
Purity 95% 90% 95% 90% by HPLC

Features

  • More informative, reliable and convenient than zymography, DQ collagen, SHG, and TEM
  • High affinity and unparalleled specificity to collagen with essentially no nonspecific binding
  • Applicable to all types of collagen from all species, relying on collagen's secondary structure instead of any defined sequence for binding
  • Suitable for both frozen and paraffin-embedded sections with no need for antigen retrieval
  • A non-antibody approach with no species restrictions against any co-staining antibody
  • Small size (2% of IgG by MW) enabling facile tissue penetration and whole specimen staining without sectioning
  • Stable in solution under 4 °C, eliminating the need to aliquot for storage
  • Now suitable for in vivo studies
  CHP Collagen antibodies C1, 2C antibody In situ zymography SHG TEM
Detecting denatured collagen  
A direct method        
Applicable to multiple collagen types          
Applicable to mm-sized tissue samples      
No control test needed      
No advanced instrument or specialised skill needed          

Applications

  1. Histopathology - CHP marks tissue damage and remodelling under many pathologic and physiologic events through binding to degraded collagen.
  2. Collagen Identification - CHP visualises collagen bands of all types in SDSPAGE with high specificity.
  3. Mechanical Damage - CHP enables measuring and localising mechanical injury to collagenous tissue at the molecular level in almost all possible tissue types, including bone, cartilage, tendon, ligament, intervertebral discs, heart valves, blood vessels, skin, cornea and more.
  4. Tissue Decellularisation - CHP allows direct and quantitative assessment of denatured collagen in the ECM materials decellularized with different detergents and protocols.

Collagen Mimetic Peptides

Prof. Michael Yu describes examples of the vast potential medical applications of CHP, which was previously termed collagen mimetic peptide.

Blog posts

Analytical Tools

All the analytical tools you need for your research: in vivo products, tetramers, ELISAs, small molecules & proteins (RUO & GMP)!

More

Understanding ECM Remodelling in Full-Thickness Skin Grafts: Insights from Collagen Hybridising Peptide Staining

Using CHP staining, researchers observed active collagen turnover in grafts, indicating that the ECM was being actively remodelled.

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Advancing Intervertebral Disc Degeneration Assessment with Collagen Hybridising peptides: A Fast, Accurate Approach for Research and Clinical Applications

Collagen Hybridising Peptides (CHPs) selectively bind to damaged collagen, allowing precise visualisation and quantification of IVD in tissue samples.

More

Using Collagen Hybridising Peptides to Improve IPF Diagnosis and Imaging

This study uses collagen hybridising peptides (CHPs) to bind specifically to denatured collagen and detect fibrosis.

More

Documents

  Category Title  
What are CHPs for in vivo?Peptides DocumentsWhat are CHPs for in vivo?  View
3Helixs Collagen Hybridising Peptide (CHP) is a synthetic peptide that can specifically bind to denatured collagen by structural recognition of exposed alpha-strands. Their in vivo CHP is conjugated to a near-infrared dye to enable accurate and reliable measurements of collagen turnover in live animal models.  
What are CHPs?Peptides DocumentsWhat are CHPs?  View
CHPs are unique collagen-targeting peptides that only bind to denatured collagen. 3Helixs Collagen Hybridising Peptide is a synthetic peptide that can specifically bind to denatured collagen through hydrogen bonding.  
Quantifying Collagen Content in Histopathology by Fluorescent CHP HybridisationPeptides DocumentsQuantifying Collagen Content in Histopathology by Fluorescent CHP Hybridisation  View
Whitepaper by Dr. Lucas Bennink and Dr. Yang Li 3Helix Inc. Salt Lake City, Utah, USA. 84108  
Determining Total Collagen Content: Stain Comparison B-CHP vs. Massons Trichrome vs. Picrosirius Red vs. Herovicis StainPeptides DocumentsDetermining Total Collagen Content: Stain Comparison B-CHP vs. Massons Trichrome vs. Picrosirius Red vs. Herovicis Stain  View
Whitepaper from Lucas Bennink, Ph.D. and Julian Kessler, Ph.D. 3Helix Inc. Salt Lake City, Utah, USA. 84108  
Collagen Hybridising Peptides (CHPs) in Multiple Myeloma ResearchPeptides DocumentsCollagen Hybridising Peptides (CHPs) in Multiple Myeloma Research  View
Collagen Hybridising Peptides offer a powerful and direct approach to address critical areas of Multiple Myeloma research by specifically visualising and quantifying structural collagen changes.  
CHP User GuidesDatasheets DocumentsCHP User Guides  View
User Guides: Damaging Collagen Histological Staining with CHPs, SDS-PAGE/In-Gel Western with CHPs and Histological Staining with CHPs.  
3Helix Imaging Fibrotic Conditions with Collagen Hybridising PeptidesPeptides Documents3Helix Imaging Fibrotic Conditions with Collagen Hybridising Peptides  View
CHP Applications - Fibrotic Conditions  
3Helix Visualising Collagen Remodelling in Skeletal Tissues with Collagen Hybridising PeptidesPeptides Documents3Helix Visualising Collagen Remodelling in Skeletal Tissues with Collagen Hybridising Peptides  View
CHP Applications - Skeletal Disease and Development  
Peptides Videos3Helix - Fibrotic Tissue Staining with CHP  View
Collagen hybridizing peptides allow for visualization of fibrotic tissue or any tissues with an increase of collagen turnover.  
Peptides Videos3Helix - Assessing Mechanical Damage of Collagen with CHPs  View
Collagen is the major building block in all load bearing tissues including tendon, ligament, bone, and cartilage. Mechanical injury to these tissues causes changes in collagen structure which can initiate pathological processes without detectable morphological changes at the macroscale. Using CHPs you can now visualize the mechanical damage to the triple helix.  
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