Lipoyl-TRIM21 (human) (rec.) (His)

E3 Ubiquitin-protein Ligase TRIM21; 52 kDa Ro Protein; Tripartite Motif-containing Protein 21

0.1mg/ml after reconstitution.

32160000

<0.01EU/µg purified protein (LAL test).

liquid

Lyophilized. Contains 50mM Tris-HCl, pH 8 and 300mM NaCl.

After reconstitution, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.Centrifuge lyophilized vial before opening and reconstitution.PBS containing at least 0.1% BSA should be used for further dilutions.

Recombinant protein. Human TRIM21 (aa 1-475) is fused at the N-terminus to a Lipoyl domain (bacterial pyruvate dehydrogenase) and at the C-terminus to a 6xHis-tag. Source/Host: E. coli. Purity: >90% (SDS-PAGE). Lyophilized. Contains 50mM Tris-HCl, pH 8 and 300mM NaCl. TRIM21 (tripartite motif-containing protein 21) is a cytosolic Fc receptor induced by interferon (IFN). TRIM21 is distinguished from other Fc receptors by showing broad antibody specificity as it can activate its functions upon binding to IgG, IgM as well as IgA while other Fc receptors display more restricted antibody isotype and subtype specificities. TRIM21 also functions as a E3 ligase. During infection, antibodies are delivered efficiently to the cytosol when bound to intracellular pathogens such as viruses and bacteria. The antibody-pathogen complex in the cytosol upon engagement of the protein TRIM21 is ubiquitinylated and degraded by the proteasome machinery. Trim-Away is a method to degrade endogenous proteins based on TRIM21, Fc receptor binding and ubiquitin ligase activity. TRIM21, which recognizes antibody-bound proteins of interest, targets them for degradation by the proteasome. The Lipoyl-TRIM21 (human) (His) (rec.) protein is used in the original papers describing Trim-Away.

~68kDa

P19474

Plastic Vial

TRIM21 (tripartite motif-containing protein 21) is a cytosolic Fc receptor induced by interferon (IFN). TRIM21 is distinguished from other Fc receptors by showing broad antibody specificity as it can activate its functions upon binding to IgG, IgM as well as IgA while other Fc receptors display more restricted antibody isotype and subtype specificities. TRIM21 also functions as a E3 ligase. During infection, antibodies are delivered efficiently to the cytosol when bound to intracellular pathogens such as viruses and bacteria. The antibody-pathogen complex in the cytosol upon engagement of the protein TRIM21 is ubiquitinylated and degraded by the proteasome machinery. Trim-Away is a method to degrade endogenous proteins based on TRIM21, Fc receptor binding and ubiquitin ligase activity. TRIM21, which recognizes antibody-bound proteins of interest, targets them for degradation by the proteasome. The Lipoyl-TRIM21 (human) (His) (rec.) protein is used in the original papers describing Trim-Away.

>90% (SDS-PAGE)

Human TRIM21 (aa 1-475) is fused at the N-terminus to a Lipoyl domain (bacterial pyruvate dehydrogenase) and at the C-terminus to a 6xHis-tag.

E. coli

His

Non-hazardous

Other Proteins

12352202

Stable for at least 6 months after receipt when stored at -20°C.Working aliquots are stable for up to 3 months when stored at -20°C.