HSPB1 Antibody, KO Validated

Back Contact us
ProSci
Product Code: PSI-16-557
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-16-557-50uL50uL£505.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 6
Western blot analysis of extracts from normal (control) and HSPB1 knockout (KO) HeLa cells, using HSPB1 antibody (16-557) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
2 / 6
Immunohistochemistry of paraffin-embedded human breast cancer using HSPB1 antibody (16-557) at dilution of 1:100 (40x lens).
3 / 6
Immunohistochemistry of paraffin-embedded rat ovary using HSPB1 antibody (16-557) at dilution of 1:100 (40x lens).
4 / 6
Immunohistochemistry of paraffin-embedded rat brain using HSPB1 antibody (16-557) at dilution of 1:100 (40x lens).
5 / 6
Immunohistochemistry of paraffin-embedded mouse kidney using HSPB1 antibody (16-557) at dilution of 1:100 (40x lens).
6 / 6
Immunofluorescence analysis of HeLa cells using HSPB1 antibody (16-557) at dilution of 1:100. Blue: DAPI for nuclear staining.
❮❯

Western blot analysis of extracts from normal (control) and HSPB1 knockout (KO) HeLa cells, using HSPB1 antibody (16-557) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human breast cancer using HSPB1 antibody (16-557) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat ovary using HSPB1 antibody (16-557) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using HSPB1 antibody (16-557) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using HSPB1 antibody (16-557) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using HSPB1 antibody (16-557) at dilution of 1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
HSPB1, CMT2F, DKFZp586P1322, HS.76067, HSP27, HSP28, Hsp25, HMN2B, SRP27, HEL-S-102
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human HSPB1 (NP_001531.1).
NCBI Gene ID #:
3315
NCBI Official Name:
heat shock 27kDa protein 1
NCBI Official Symbol:
HSPB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 25kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle, Signal Transduction, Stem Cell
Swissprot #:
P04792
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Antibody is Knockout validated.