GZMA Antibody

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ProSci
Product Code: PSI-22-069
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-22-069-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using GZMA antibody (22-069) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunofluorescence analysis of U2OS cells using GZMA antibody (22-069) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using GZMA antibody (22-069) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of U2OS cells using GZMA antibody (22-069) at dilution of 1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
GZMA, granzyme A, Hanukah factor serine protease), Granzyme A (Cytotoxic T-lymphocyte-associated serine esterase-3, CTL tryptase, HFSP, CTLA3, granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3), GZMA
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here is a T cell- and natural killer cell-specific serine protease that may function as a common component necessary for lysis of target cells by cytotoxic T lymphocytes and natural killer cells.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 29-262 of human GZMA (NP_006135.1).
NCBI Gene ID #:
3001
NCBI Official Name:
granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3)
NCBI Official Symbol:
GZMA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 35kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Immunology, Innate Immunity
Swissprot #:
P12544
User NOte:
Optimal dilutions for each application to be determined by the researcher.