LAG3 Detection Set

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ProSci
Product Code: PSI-SD8700
Supplier: ProSci
CodeSizePrice
PSI-SD8700-1Set1Set£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Regulatory Status: RUO
Shipping:
Dry Ice
Storage:
LAG3 antibodies should be stored in working aliquots at -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Documents

Further Information

Background:
The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1), negatively regulating T-cell function and homeostasis (2). It is expressed in B, T, and NK cells, monocytes, and dendritic cells (3), and acts to regulate T cell expansion (4). LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy.
Background References:
  • Huard B, Tournier M, Hercend T, et al. Lymphocyte-activation gene 3/major histocompatibility complex class II interaction modulates the antigenic response of CD4+ T lymphocytes. Eur J Immunol 1994; 24:3216?21.
  • Triebel F. LAG-3: a regulator of T-cell and DC responses and its use in therapeutic vaccination. Trends Immunol 2003; 24:619?22.
  • Workman CJ, Wang Y, El Kasmi KC, et al. LAG-3 regulates plasmacytoid dendritic cell homeostasis. J Immunol. 2009; 182:1885?91.
  • Workman CJ, Vignali DA. The CD4-related molecule, LAG-3 (CD223), regulates the expansion of activated T cells. Eur J Immunol 2003; 33:970?9.
Buffer:
LAG3 Antibodies are supplied in PBS.
Concentration:
Antibody 1 mg/mL
Conjugate:
Unconjugated
Immunogen:
LAG3 antibodies were raised against a recombinant protein corresponding to amino acids 23-434 of human LAG3.
Physical State:
Liquid
Purification:
LAG3 Antibodies are affinity chromatography purified via Nickel column. Antibodies are supplied as a His-tagged purified protein.??They also contains a myc-tag for detection.
Set Contents:
20 μg:
LAG-3 Antibody [2A11], Cat. No. SD8837
LAG-3 Antibody [1A6], Cat. No. SD8839
LAG-3 Antibody [2B11], Cat. No. SD8841
LAG-3 Antibody [1C6], Cat. No. SD8842
LAG-3 Antibody [1A8], Cat. No. SD8843

100 μg:
cMyc-tag Antibody [5G5H7], Cat. No. PM-7669
User Note:
Optimal dilutions for each application to be determined by the researcher.

References

  1. Huard B, Tournier M, Hercend T, et al. Lymphocyte-activation gene 3/major histocompatibility complex class II interaction modulates the antigenic response of CD4+ T lymphocytes. Eur J Immunol 1994; 24:3216?21.
  2. Triebel F. LAG-3: a regulator of T-cell and DC responses and its use in therapeutic vaccination. Trends Immunol 2003; 24:619?22.
  3. Workman CJ, Wang Y, El Kasmi KC, et al. LAG-3 regulates plasmacytoid dendritic cell homeostasis. J Immunol. 2009; 182:1885?91.
  4. Workman CJ, Vignali DA. The CD4-related molecule, LAG-3 (CD223), regulates the expansion of activated T cells. Eur J Immunol 2003; 33:970?9.