anti-FADD (human) mAb (1C4) (Biotin)
| Code | Size | Price |
|---|
| AG-20B-0080B-C100 | 100 ug | £270.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Shipping:
Blue Ice
Storage:
-20°C
Further Information
Alternate Names/Synonyms:
FAS-associated Death Domain Protein; Growth-inhibiting Gene 3 Protein; Mediator of Receptor Induced Toxicity; MORT1
Concentration:
1 mg/ml
EClass:
32160000
Form (Short):
liquid
Formulation:
Liquid. In PBS and 0.02% sodium azide.
Handling Advice:
After opening, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.
Immunogen:
Recombinant human FADD.
Labels - Conjugates:
Biotin
Long Description:
Monoclonal Antibody. Recognizes the C-terminus of human FADD only in its unphosphorylated form. Application: ELISA,IP,WB. Clone: 1C4. Isotype: Mouse IgG1. Liquid. In PBS and 0.02% sodium azide. FADD (FAS-associated death domain protein) is an apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. Procaspase-8 belongs to the family of caspases. Binding of FasL to Fas leads to formation of a receptor complex at the cellular membrane, which was named DISC. The DISC consists of oligomerized receptors, the DD-containing adaptor molecule FADD, procaspase-8, procaspase-10 and c-FLIP. The DISC structure provides a platform for the oligomerization of procaspase-8 that allows two procaspase-8 homodimers to be in the close proximity leading to the initial activation of procaspase-8. FADD is also involved in interferon-mediated antiviral immune response and functions in the positive regulation of interferon signaling. FADD also plays a role in embryonic development and the cell cycle reentry of T cells.
NCBI, Uniprot Number:
Q13158
Package Type:
Plastic Vial
Product Description:
FADD (FAS-associated death domain protein) is an apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. Procaspase-8 belongs to the family of caspases. Binding of FasL to Fas leads to formation of a receptor complex at the cellular membrane, which was named DISC. The DISC consists of oligomerized receptors, the DD-containing adaptor molecule FADD, procaspase-8, procaspase-10 and c-FLIP. The DISC structure provides a platform for the oligomerization of procaspase-8 that allows two procaspase-8 homodimers to be in the close proximity leading to the initial activation of procaspase-8. FADD is also involved in interferon-mediated antiviral immune response and functions in the positive regulation of interferon signaling. FADD also plays a role in embryonic development and the cell cycle reentry of T cells.
Purity:
>95% (SDS-PAGE)
Source / Host:
Purified from concentrated hybridoma tissue culture supernatant.
Specificity:
Recognizes the C-terminus of human FADD only in its unphosphorylated form.
Transportation:
Non-hazardous
UNSPSC Category:
Primary Antibodies
UNSPSC Number:
12352203
Use & Stability:
Stable for at least 1 year after receipt when stored at -20°C.
References
DEDD, a novel death effector domain-containing protein, targeted to the nucleolus: A.H. Stegh, et al.; EMBO J. 17, 5974 (1998) | Phosphorylation of FADD/ MORT1 at serine 194 and association with a 70-kDa cell cycle-regulated protein kinase: C. Scaffidi, et al.; J. Immunol. 164, 1236 (2000) | Phosphorylation of FADD at Serine 194 by CKIalpha Regulates Its Nonapoptotic Activities: E.C. Alappat, et al.; Mol. Cell 19, 321 (2005) | CD95 Stimulation Results in the Formation of a Novel Death Effector Domain Protein-containing Complex: I.N. Lavrik, et al.; J. Biol. Chem. 283, 26401 (2008) | A New C-Terminal Cleavage Product of Procaspase-8, p30, Defines an Alternative Pathway of Procaspase-8 Activation: J.C. Hoffmann, et al.; Mol. Cell. Biol. 29, 4431 (2009) | Caspase-8 activity has an essential role in CD95/Fas-mediated MAPK activation: A.M.M. Kober, et al.; Cell Death Dis. 2, e212 (2011) | Modulation of the CD95-Induced Apoptosis: The Role of CD95 N-Glycosylation: O.M. Shatnyeva, et al.; PLoS One 6, e19927 (2011) | Quantification of High-Molecular Weight Protein Platforms by AQUA Mass Spectrometry as Exemplified for the CD95 Death-Inducing Signaling Complex (DISC): U. Warnken, et al.; Cells 2, 476 (2013) | Targeting Thioredoxin-1 by dimethyl fumarate induces ripoptosome-mediated cell death: A. Schroeder, et al.; Sci. Rep. 7, 43168 (2017) | A Dual Role of Caspase-8 in Triggering and Sensing Proliferation-Associated DNA Damage, a Key Determinant of Liver Cancer Development: Y. Boege, et al.; Cancer Cell 32, 342 (2017) | Delineating the role of c-FLIP/NEMO interaction in the CD95 network via rational design of molecular probes: N.V. Ivanisenko, et al.; BMC Genomics 20, 293 (2019)