Ac-Trp-Leu-Ala-AMC
| Code | Size | Price |
|---|
| AG-CP3-0035-M001 | 1 mg | £64.00 |
Quantity:
| AG-CP3-0035-M005 | 5 mg | £227.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
Ambient
Storage:
-20°C
Further Information
Alternate Names/Synonyms:
Ac-WLA-AMC; Proteasome Substrate
Appearance:
Lyophilized powder.
CAS:
1104011-59-7
EClass:
32160000
Form (Short):
liquid
Handling Advice:
Avoid freeze/thaw cycles.Protect from light.
InChi:
InChI=1S/C32H37N5O6/c1-17(2)12-26(37-32(42)27(35-20(5)38)14-21-16-33-25-9-7-6-8-24(21)25)31(41)34-19(4)30(40)36-22-10-11-23-18(3)13-29(39)43-28(23)15-22/h6-11,13,15-17,19,26-27,33H,12,14H2,1-5H3,(H,34,41)(H,35,38)(H,36,40)(H,37,42)/t19-,26-,27-/m0/s1
InChiKey:
PIJMRHCPKXLMIS-DOYMOWJKSA-N
Long Description:
Chemical. CAS: 1104011-59-7. Formula: C32H37N5O6. MW: 587.7. Synthetic. Fluorogenic peptide substrate for measuring chymotrypsin-like activity of the 20S proteasome. This substrate is hydrolyzed by the beta5-subunit of the 20S proteasome to generate detectable fluorescence. 20S Proteasome enzyme requires activation with 0.035% SDS in the assay buffer. Excitation: 345nm. Emission: 445nm. Specific substrate to the constitutive proteasome, not hydrolyzed efficiently by the immunoproteasome. This substrate is useful for inhibitor screening and kinetic analysis.
Molecular Formula:
C32H37N5O6
Molecular Weight:
587.7
Other data:
Use: After preparing a stock solution in DMSO (>10mM) store product at -20°C to -80°C. It is recommended to make multiple aliquots after the first thaw to ensure best performance. Chymotrypsin-like activity can be measured using a typical working concentration range from 10-50µM.
Package Type:
Plastic Vial
Product Description:
Fluorogenic peptide substrate for measuring chymotrypsin-like activity of the 20S proteasome. This substrate is hydrolyzed by the beta5-subunit of the 20S proteasome to generate detectable fluorescence. 20S Proteasome enzyme requires activation with 0.035% SDS in the assay buffer. Excitation: 345nm. Emission: 445nm. Specific substrate to the constitutive proteasome, not hydrolyzed efficiently by the immunoproteasome. This substrate is useful for inhibitor screening and kinetic analysis.
Purity:
>98% (HPLC)
Sequence:
Acetyl-Trp-Leu-Ala-7-amido-4-methylcoumarin
SMILES:
CC(C1=CC=C(C=C1O2)NC([C@H](C)NC([C@H](CC(C)C)NC([C@@H](NC(C)=O)CC3=CNC4=CC=CC=C34)=O)=O)=O)=CC2=O
Solubility Chemicals:
Soluble in DMSO.
Transportation:
Non-hazardous
UNSPSC Category:
Biochemical Reagents
UNSPSC Number:
12352200
Use & Stability:
Stable for at least 1 year after receipt when stored at -20°C.
References
Characterization of a new series of non-covalent proteasome inhibitors with exquisite potency and selectivity for the 20S beta5-subunit: C. Blackburn, et al.; Biochem. J. 430, 461 (2010) | The immunoproteasome as a therapeutic target for hematological malignancies: Z. Miller, et al.; Curr. Can. Drug Targets 14, 537 (2014) | Immunoproteasome-selective inhibitors: a promising strategy to treat hematologic malignancies, autoimmune and inflammatory diseases: R. Ettari, et al.; Curr. Med. Chem. 23, 1217 (2016)