hRIP3 Antibody

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ProSci
Product Code: PSI-8963
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-8963-0.02mg0.02mg£150.00
Quantity:
PSI-8963-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
blue ice or RT
Storage:
hRIP3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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<strong>Figure 1 Western Blot Validation in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: hRIP3, 8963 (0.5 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 2 Western Blot Validation in Human Tissues</strong><br> Loading: 15 μg of lysates per lane. Antibodies: hRIP3, 8963 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 3 Western Blot Validation of hRIP3 in Human THP-1 Cell Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: hRIP3, 8963 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1-2: human THP-1 cell lysate in the absence (Lane 1) or the presence (Lane 2) of blocking peptide.
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<strong>Figure 4 Immunofluorescence Validation of hRIP3 in Molt4 Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed Molt4 cells labeling hRIP3 with 8963 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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<strong>Figure 5 Immunofluorescence Validation of hRIP3 in Human Breast Tissue</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed human breast tissue labeling hRIP3 with 8963 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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<strong>Figure 6 Immunofluorescence Validation of hRIP3 in Human Colon Tissue</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed human colon tissue labeling hRIP3 with 8963 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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<strong>Figure 7 Immunohistochemistry Validation of hRIP3 in Human Liver Tissue</strong><br> Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-hRIP3 antibody (8963) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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<strong>Figure 8 Immunohistochemistry Validation of hRIP3 in Human Breast Tissue</strong><br> Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-hRIP3 antibody (8963) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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<strong>Figure 9 Immunohistochemistry Validation of hRIP3 in Human Colon Tissue</strong><br> Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-hRIP3 antibody (8963) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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<strong>Figure 1 Western Blot Validation in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: hRIP3, 8963 (0.5 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Western Blot Validation in Human Tissues</strong><br> Loading: 15 μg of lysates per lane. Antibodies: hRIP3, 8963 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Western Blot Validation of hRIP3 in Human THP-1 Cell Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: hRIP3, 8963 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1-2: human THP-1 cell lysate in the absence (Lane 1) or the presence (Lane 2) of blocking peptide.
<strong>Figure 4 Immunofluorescence Validation of hRIP3 in Molt4 Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed Molt4 cells labeling hRIP3 with 8963 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 5 Immunofluorescence Validation of hRIP3 in Human Breast Tissue</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed human breast tissue labeling hRIP3 with 8963 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 6 Immunofluorescence Validation of hRIP3 in Human Colon Tissue</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed human colon tissue labeling hRIP3 with 8963 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 7 Immunohistochemistry Validation of hRIP3 in Human Liver Tissue</strong><br> Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-hRIP3 antibody (8963) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 8 Immunohistochemistry Validation of hRIP3 in Human Breast Tissue</strong><br> Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-hRIP3 antibody (8963) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 9 Immunohistochemistry Validation of hRIP3 in Human Colon Tissue</strong><br> Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-hRIP3 antibody (8963) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Documents

Further Information

Additional Names:
hRIP3 Antibody: Rip3, AW107945, 2610528K09Rik, RIP-like protein kinase 3, RIP-3
Application Note:
WB: 0.5-1 μg/mL; IF: 20 μg/mL; IHC: 2-5 μg/mL.

Antibody validated: Western Blot, Immunofluorescence and Immunohistochemistry in human samples. All other applications and species not yet tested.
Background:
hRIP3 Antibody: Certain serine/threonine protein kinases, such as ASK1, RIP, DAP, and ZIP kinases, are mediators of apoptosis. Receptor interacting proteins including RIP and RIP2/RICK mediate apoptosis induced by TNFR1 and Fas, two prototype members in the death receptor family. A novel member in the RIP kinase family was recently identified and designated RIP3. RIP3 contains N-terminal kinase domain but, unlike RIP or RIP2, lacks the C-terminal death or CARD domain. RIP3 binds to RIP and TNFR1, mediates TNFR1 induced apoptosis, and attenuates RIP and TNFR1 induced NF-?B activation. Overexpression of RIP3 induces apoptosis and NF-?B activation. The messenger RNA of RIP3 is expressed in a subset of adult tissues.
Background References:
  • Yu et al. Curr Biol. 1999;9(10):539-42.
  • Sun et al. J Biol Chem. 1999;274(24):16871-5.
  • Pazdernik et al. Mol Cell Bio. 1999; 19(10):6500-8 (WD0102)
Buffer:
hRIP3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Anti-hRIP3 antibody (8963) was raised against a peptide corresponding to 18 amino acids near the carboxy terminus of human hRIP3.

The immunogen is located within the last 50 amino acids of hRIP3.
ISOFORMS:
Human RIP3 has 3 isoforms, including isoform 1 (518aa, 57kD), isoform 2 (252aa, 28kD) and isoform 3 (231aa, 25kD). Mouse RIP3 has one isoform (486aa, 53kD) and Rat RIP3 also has one isoform (478aa, 52kD). 8963 can detect only human isoform 1 and cannot detect other human isoforms, mouse and rat isoforms.
NCBI Gene ID #:
11035
NCBI Official Name:
receptor-interacting serine-threonine kinase 3
NCBI Official Symbol:
Ripk3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 57kD

Observed: 57 kD
Protein Accession #:
Q9Y572
Protein GI Number:
205371831
Purification:
hRIP3 Antibody is Protein A purified.
Research Area:
Apoptosis
SPECIFICITY:
Several isoforms of hRIP3 are known to exist.
Swissprot #:
Q9Y572
User NOte:
Optimal dilutions for each application to be determined by the researcher.