HpARI (CCP1/2) (rec.) (His)
| Code | Size | Price |
|---|
| AG-40B-0201-C050 | 50 ug | £340.00 |
Quantity:
| AG-40B-0201-3050 | 3 x 50 ug | £670.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Shipping:
BLUE ICE
Storage:
Short Term: +4°C, Long Term: -20°C
Documents
Further Information
Alternate Names/Synonyms:
Heligmosomoides Polygyrus Alarmin Release Inhibitor Truncated (CCP1/2); HpARI Truncation Lacking CCP3
Biological Activity:
Enhances activity of mouse or human IL-33 in vivo and in vitro.
Concentration:
After reconstitution: 1mg/ml
EClass:
32160000
Endotoxin:
<0.01EU/µg purified protein (LAL test).
Form (Short):
liquid
Formulation:
Lyophilized. Contains PBS.
Handling Advice:
After reconstitution, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.PBS containing at least 0.1% BSA should be used for further dilutions.
Long Description:
Recombinant protein. Heligmosomoides polygyrus protein HpARI (aa 17-165) is fused at the C-terminus to a His-tag. Binds to human and mouse IL-33. Endotoxin: <0.01EU/µg purified protein (LAL test). Lyophilized. Contains PBS. HpARI is a protein secreted by the mouse parasite Heligmosomoides polygyrus. The mature protein HpARI, containing three predicted Complement Control Protein (CCP)-like modules (also known as Short Consensus Repeats (SCRs) or sushi-domains), suppresses type 2 (allergic) immune responses through interference in the interleukin-33 (IL-33) pathway. During cell damage, HpARI gains access to the nucleus of necrotic cells, where it binds directly to IL-33 and nuclear DNA, preventing secretion and binding of IL-33 to its receptor. A non-natural truncation consisting of the first 2 domains of HpARI (CCP1/2) retains IL-33 and DNA binding capacity. HpARI (CCP1/2) is able to stabilize IL-33, increasing its half-life and amplifying its effects. HpARI (CCP1/2) increases IL-33 activity by protecting it from oxidation and proteolytic degradation. HpARI (CCP1/2) (rec.) (His) is a new type of reagent to study IL-33-mediated pathology in vivo.
Molecular Weight:
~28kDa (SDS-PAGE)
NCBI, Uniprot Number:
Worm base HPBE_0000813301
Package Type:
Plastic Vial
Product Description:
HpARI is a protein secreted by the mouse parasite Heligmosomoides polygyrus. The mature protein HpARI, containing three predicted Complement Control Protein (CCP)-like modules (also known as Short Consensus Repeats (SCRs) or sushi-domains), suppresses type 2 (allergic) immune responses through interference in the interleukin-33 (IL-33) pathway. During cell damage, HpARI gains access to the nucleus of necrotic cells, where it binds directly to IL-33 and nuclear DNA, preventing secretion and binding of IL-33 to its receptor. A non-natural truncation consisting of the first 2 domains of HpARI (CCP1/2) retains IL-33 and DNA binding capacity. HpARI (CCP1/2) is able to stabilize IL-33, increasing its half-life and amplifying its effects. HpARI (CCP1/2) increases IL-33 activity by protecting it from oxidation and proteolytic degradation. HpARI (CCP1/2) (rec.) (His) is a new type of reagent to study IL-33-mediated pathology in vivo.
Purity:
>90% (SDS-PAGE)
Sequence:
Heligmosomoides polygyrus protein HpARI (aa 17-165) is fused at the C-terminus to a His-tag.
Source / Host:
HEK 293 cells
Specificity:
Binds to human and mouse IL-33.
TAGs:
His
Transportation:
Non-hazardous
UNSPSC Category:
Interleukins
UNSPSC Number:
41116127
Use & Stability:
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
References
An IL-1 cytokine member, IL-33, induces human basophil activation via its ST2 receptor: M. Suzukawa, et al.; J. Immunol. 181, 5981 (2008) | HpARI protein secreted by a helminth parasite suppresses interleukin-33: M. Osbourne, et al.; Immunity 47, 739 (2017) | A Truncated Form of HpARI Stabilizes IL-33, Amplifying Responses to the Cytokine. Chauch? C. et al. Frontiers in Immunology, 11, 1363 (2020)