SARS-CoV-2 (COVID-19) Spike G142D Δ143-145VYY Antibody (Omicron)

Product Code: PSI-9793

Supplier: ProSci

Code	Size	Price

PSI-9793-0.02mg

0.02mg

£150.00

Quantity:

PSI-9793-0.1mg

0.1mg

£449.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit

Antibody Isotype: IgG

Antibody Clonality: Polyclonal

Regulatory Status: RUO

Target Species: SARS-CoV-2

Applications:

Enzyme-Linked Immunosorbent Assay (ELISA)
Western Blot (WB)

Storage:

SARS-CoV-2 Omicron Variant Spike antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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<strong>Figure 1 Immunohistochemistry Validation of Omicron Variant Spike Specific Antibody on a Nasal Swab Sample from a Omicron COVID-19 Patient.</strong><br>
Immunohistochemical analysis of 4% paraformaldehyde-fixed cells from a nasal swab from a patient infected with the Omicron variant stained with Omicron specific antibody (9793) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

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<strong>Figure 2 Immunofluorescence Validation of Omicron Variant Spike Specific Antibody on a Nasal Swab Sample from a Patient Infected with the Omicron Variant. </strong><br>
Immunofluorescent analysis of 4% paraformaldehyde-fixed patient nasal swab sample labeling spike with 9793 and nucleocapsid with 35-722 at 10 μg/mL for each, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution for spike (9793,green) and goat anti-mouse IgG secondary antibody at 1/500 dilution for nucleocapsid (35-722, red) with DAPI counter staining (blue).

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<strong>Figure 3 SARS-CoV-2 Omicron Variant Spike Antibodies Specifically Detect Omicron Variant Spike S1 Protein in an ELISA </strong><br>
Coating Antigen: SARS-CoV-2 spike S1 proteins of WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and omicron variant (B.1.1.529), 1 μg/mL, incubated at 4 ˚C overnight.
Detection Antibodies: SARS-CoV-2 Omicron Variant Spike antibody, 9793, dilution: 200 and 1000 ng/mL, incubated at RT for 1 hr.
Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr.

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<strong>Figure 4 WB Validation of of Omicron Variant Spike Antibodies with SARS-CoV-2 Omicron Variant Spike S1 Protein </strong><br>
Loading: 30 ng of SARS-CoV-2 spike S1 proteins, including WT, Delta variant (B.1.617.2) and Omicron variant (B.1.1.529).
Detection Antibodies: SARS-CoV-2 Omicron Variant Spike antibody, 9793, 2 μg/mL, incubated at RT for 1 hr.
Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr.
<strong>SARS-CoV-2 Omicron Variant Spike antibody (9793) specifically detects Omicron variant spike S1 protein (95-129), but not spike S1 protein of WT (10-300) and Delta (95-127). </strong>

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<strong>Figure 5 ELISA Validation of Omicron Variant Spike S1 Antibodies with SARS-CoV-2 Omicron Variant Spike S1 Protein </strong><br>
Coating Antigen: SARS-CoV-2 spike S1 proteins, WT and Omicron variant, 1 μg/mL, incubated at 4 ˚C overnight.
Detection Antibodies: SARS-CoV-2 Omicron spike antibody, 9793, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr.
Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr.
<strong>SARS-CoV-2 Omicron spike antibody specifically detects spike S1 protein of Omicron variant (95-129), but not WT (10-300). </strong>

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<strong>Figure 6 WB Validation of of Omicron Variant Spike Antibodies with SARS-CoV-2 Variant Spike S1 Proteins </strong><br>
Loading: 30 ng of SARS-CoV-2 spike S1 proteins, including WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and omicron variant (B.1.1.529),
Detection Antibodies: SARS-CoV-2 Omicron Variant Spike antibody, 9793, 2 μg/mL, incubated at RT for 1 hr.
Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr.
<strong>SARS-CoV-2 Omicron Variant Spike antibody (9793) specifically detects Omicron variant spike S1 protein (95-129), but not spike S1 protein of WT (10-300) and all other variants. </strong>

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Documents

Further Information

Additional Names:

SARS-CoV-2 Omicron Variant Spike antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Surface Glycoprotein, Spike protein, Omicron Variant

Application Note:

E, WB

Background:

SARS-CoV-2 Omicron variant, a variant of concern (VOC), known as B.1.1.529, was detected in South Africa at the end of November in 2021. However, it rapidly spread all over of the world and now it is the dominant variant in the world, which account for more than 90% of the new cases. Omicron variant spike protein carries around 30 amino acid changes, including mutations, deletions and insertions, 15 of which are in the receptor binding domain (RBD). Enhanced transmission of the Omicron variant was observed globally, which is at least 70 times more contagious than the other variants. The Omicron variant affects the effectiveness of COVID-19 vaccine and is resistant to neutralization (monoclonal antibody treatments) to a large extent.

Background References:

Gowrisankar et al. European Physical Journal Plus, 2022, 137(1), 100.
Zhang et al. PNAS of U.S.A. 2021, 118(47):e2109905118.
Cameroni et al. Nature 2021.
Rossler et al. NEJM 2022.
Muik et al. Science 2022.

Buffer:

Anti-SARS-CoV-2 Omicron Variant Spike antibody is supplied in PBS containing 0.02% sodium azide.

Concentration:

1 mg/mL

Conjugate:

Unconjugated

Homology:

Predicted reactivity based on immunogen sequence: SARS-CoV Spike proteins: (33.3%)

Immunogen:

Anti-SARS-CoV-2 Omicron variant specific Spike (G142D Δ143-145VYY) antibody(9793) was raised against a peptide corresponding to 12 amino acids near the amino terminal domain of SARS-CoV-2 Omicron variant (B.1.1.529) Spike protein.

The immunogen is located within 130-180 amino acids of SARS-CoV-2 Omicron variant (B.1.1.529) Spike protein.

NCBI Gene ID #:

43740568

NCBI Official Name:

surface glycoprotein

NCBI Official Symbol:

NCBI Organism:

Severe acute respiratory syndrome coronavirus 2

Physical State:

Liquid

Protein Accession #:

QHD43416

Protein GI Number:

1791269090

Purification:

Anti-SARS-CoV-2 Omicron Variant Spike antibody is affinity chromatography purified via peptide column.

Research Area:

Infectious Disease, COVID-19

SPECIFICITY:

It can only detect SARS-CoV-2 Omicron Variant (B.1.1.529) Spike protein and does not cross-react with the spike proteins of other variants.

Swissprot #:

P0DTC2

User NOte:

Optimal dilutions for each application to be determined by the researcher.