Bafilomycin A1
| Code | Size | Price |
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| TAR-T6740-1mg | 1mg | £324.00 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Special offer! Add £1 to your order to get a TargetMol CCK-8 Kit. Read more here. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Quantity:
| TAR-T6740-5mg | 5mg | £687.00 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Special offer! Add £1 to your order to get a TargetMol CCK-8 Kit. Read more here. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
cool pack
Storage:
-20℃
Documents
Further Information
Bioactivity:
Bafilomycin A1 induces caspase-independent cell death in hepatocellular carcinoma cells via targeting of autophagy and MAPK pathways. Bafilomycin A1 is a vacuolar H+-ATPase inhibitor (IC50: 0.44 nM). It is also found to inhibit autophagy while induces apoptosis.Bafilomycin A1 triggers proliferative potential of senescent cancer cells in vitro and in NOD/SCID mice.
Biological Applications:
Bafilomycin A1 is a late-stage autophagy inhibitor. Autophagy, meaning "self-eating", is the cellular process of degrading its own cytoplasmic proteins and damaged organelles using lysosomes. Autophagy serves to prevent cellular damage, promote cell survival under nutrient-deficient conditions, and respond to cytotoxic stimuli. However, excessive autophagy can lead to metabolic stress, degradation of cellular components, and even cell death. Research indicates that autophagy plays a crucial role in various physiological and pathological processes such as cellular homeostasis, aging, immunity, inflammation, tumorigenesis, and neurodegenerative diseases.
CAS:
88899-55-2
Description:
Bafilomycin A1 induces caspase-independent cell death in hepatocellular carcinoma cells via targeting of autophagy and MAPK pathways. Bafilomycin A1 is a vacuolar H+-ATPase inhibitor (IC50: 0.44 nM). It is also found to inhibit autophagy while induces apoptosis. Bafilomycin A1 triggers proliferative potential of senescent cancer cells in vitro and in NOD/SCID mice.
Formula:
C35H58O9
Mechanism of Action:
Bafilomycin A1 disrupts the fusion of autophagosomes with lysosomes and the acidification of lysosomes, exerting an inhibitory effect on autophagy. The fusion of autophagosomes with lysosomes and the acidification of lysosomes are late-stage steps in the autophagic process, essential for maintaining functional autophagic flux and cellular homeostasis. Lysosomes are cellular organelles containing hydrolytic enzymes capable of degrading substances such as proteins, nucleic acids, and lipids. Modulating the acidic environment within lysosomes controls the efficiency and speed of their degradation.
Bafilomycin A1 inhibits the function of the V-ATPase within lysosomes, thereby preventing the formation of an acidic environment in lysosomes. Additionally, Baf A1 can inhibit the endoplasmic reticulum calcium ATPase Ca-P60A/SERCA, disrupting the fusion of autophagosomes with lysosomes. By acting at two steps in the autophagy, Baf A1 collectively prevents protein degradation and functional autophagy.
Molecular Weight:
622.84
Pathway:
Membrane transporter/Ion channel; Microbiology/Virology; Autophagy; Apoptosis
Purity:
0.98
Research Area:
Cellular homeostasis; Aging; Immunity; Inflammation; Tumours; Neurodegenerative diseases
SMILES:
CO[C@H]1C=CC=C(C)C[C@H](C)[C@H](O)[C@H](C)C=C(/C)C=C(OC)C(=O)O[C@@H]1[C@@H](C)[C@@H](O)[C@H](C)[C@@]1(O)C[C@@H](O)[C@H](C)[C@H](O1)C(C)C
Target:
Apoptosis; Proton pump; Antibacterial; Antibiotic; Autophagy
References
Wang J, Yan J T, Zeng S T, et al.Revealing Mitochondrion?Lysosome Dynamic Interactions and pH Variations in Live Cells with a pH-Sensitive Fluorescent Probe.Analytical Chemistry.2023
Wang J, Su Q, Wu Q, et al. Sanguinarine impairs lysosomal function and induces ROS-dependent mitophagy and apoptosis in human hepatocellular carcinoma cells. Archives of Pharmacal Research. 2021: 1-12.
Su Q, Wu Q, Chen K, et al. Induction of Estrogen Receptor ?-mediated Autophagy Sensitizes Breast Cancer Cells to TAD1822-7, a Novel Biphenyl Urea Taspine Derivative. Molecular Biology Reports. 2021
Zhang Y, Ding Y, Li M, et al. MicroRNA-34c-5p provokes isoprenaline-induced cardiac hypertrophy by modulating autophagy via targeting ATG4B. Acta Pharmaceutica Sinica B. 2021
Du L, Wu Y, Han X, et al. NICE?3?knockdown induces cell cycle arrest and autophagy in lung adenocarcinoma cells via the AKT/mTORC1 signaling pathway[J]. Experimental and Therapeutic Medicine . 2021, 21(6): 1-8.
al-Fifi ZI, et al. Insect BiochemMol Biol, 1998, 28(4), 201-211.
Du L, Wu Y, Han X, et al. NICE-3-knockdown induces cell cycle arrest and autophagy in lung adenocarcinoma cells via the AKT/mTORC1 signaling pathway. Experimental and Therapeutic Medicine. 2021, 21(6): 1-8