Anti-Human CD3 (Clone HIT3a) - Purified in vivo GOLD™ Functional Grade

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Leinco Technologies
Product Code: LEI-C2446
Product Group: Primary Antibodies
CodeSizePrice
LEI-C2446-0.5mg0.5 mg£318.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG2a κ
Antibody Clonality: Monoclonal
Antibody Clone: HIT3a
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • In Vivo Assay
Shipping:
2-8°C
Storage:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Further Information

Antigen Distribution:
CD3 is expressed on human peripheral blood lymphocytes, splenic lymphocytes, and the majority of T-CLL and T-ALL cells.
Concentration:
1.0 mg/ml
Conjugate/Tag/Label:
Purified in vivo Functional Grade
Format:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Long Description:
CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.
NCBI Gene:
916
Purity:
?95% monomer by analytical SEC, >95% by SDS Page
Target:
CD3

References

1. Meuer, S. C. et al. (1983) Nature 303:808 2. van Dongen, J. J. M. et al. (1988) Blood 71:603 3. Beverly, P. C. L. et al. (1981) Eur. J. Immunol. 11:329 4. Leukocyte Typing: 3rd Workshop: Code No. 126, 208 and 471; 4th Workshop: Paper No. T3.2