DDDDK tagged Protein PURIFICATION KIT

The ability to isolate and study a purified protein lies at the heart of modern biochemistry. Researchers in many fields require highly purified, active proteins for studies involving signaling pathways, enzymology, receptor binding, DNA binding, post-transcriptional modifications, and much more. Thus, choosing a method of purification is an important aspect in maintaining protein structure and function. Recombinant tagged protein purification methods and kits are now widely recognized. The DDDDK epitope tag (DYKDDDDK) has been widely used as a multi-purpose tag, and anti-DDDDK tag antibodies are optimally suited for identifying, detecting, purifying, and monitoring the expression levels of recombinant DDDDK tagged proteins. MBLI?s DDDDK tagged Protein PURIFICATION kit is designed for the isolation of DDDDK tagged protein from cell culture supernatants and cell lysate under neutral pH condition. Severe conditions such as acidic or alkaline elution denature protein structure. However, a neutral pH elution can preserve protein activity and native conformation. MBLI has developed the Anti-DDDDK tag Beads to purify DDDDK tagged proteins quickly and efficiently. As the Beads can be used at neutral pH, the purified proteins can maintain the activity and conformation. The elution of DDDDK tagged proteins from the Beads is achieved by the addition of the DDDDK tag peptide (DYKDDDDK). As the DDDDK tag peptide competes with DDDDK tagged proteins on the Beads, the purified proteins do not lose the protein activity. Using a Spin Column resulting in high efficiency has optimized the simple procedure of this kit.

Anti-DDDK tag beads, elution peptide, spin column sets, wash concentrate

1 year

DDDDK