APOPCYTO Caspase-3 Colorimetric Assay Kit

Product Code: MBL-4800

Supplier: MBL

Code	Size	Price

MBL-4800

100 Assays

£373.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Regulatory Status: RUO

Shipping:

dry ice

Storage:

-20°C. Please refer to datasheet for additional information

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Documents

Datasheet

Further Information

Description:

Caspases are members of the cysteine aspartic acid-specific protease family, which may be activated by a variety of signals including death receptor ligation, DNA damage, serum starvation, stress, and many more. Active caspases recognize a wide number of different substrates, leading to the activation of several different apoptotic pathways. For example, CAD (caspase-activated deoxyribonuclease), a protein involved in chromatin fragmentation into nucleosome units, is indirectly activated by caspase-3, while ICAD (inhibitor of caspase-activated deoxyribonuclease) is inactivated by caspase-3. The cleavage of several structural proteins by caspases leads to the unique apoptosis cell morphology of chromatin condensation, nucleus fragmentation and cytoplasmic integrity. Detection of caspase activity is recognized as a standard method to measure apoptosis activity. The most common method to measure caspase activity is via the fluorometric or colorimetric detection of the cleavage of caspase-specific substrates.

Gene IDs:

Human: 836 Mouse: 12367

Kit Components:

Cell Lysis Buffer, 2X Reaction Buffer, DTT, Substrate DEVD-pNA, Inhibitor DEVD-FMK, pNA (p-nitroanilide)

Shelf Life:

see label

Target:

Caspase-3

References

1). Adachi M, et al., Clin Cancer Res, 10, 3853 (2004) 2). Brigotti M, et al., Infect Immun, 75, 2201 (2007) 3). Dinnen RD, et al., J Biol Chem, 282, 26675 (2007) 4). Goga Y, et al., J Dent Res, 85, 240 (2006) 5). Ishii T, et al., Cancer Res, 65, 203 (2005) 6). Madsen-Bouterse SA, et al., Endocrinology, 147, 3826 (2006) 7). Min Y, et al., Gut, 54, 591 (2005) 8). Nozaki N, et al., Circulation, 110, 2869 (2004) 9). Numata K, et al., J Immunol, 178, 3777 (2007) 10). Sinicrope FA, et al., Clin Cancer Res, 10, 8284 (2004)