APOPCYTO Caspase-8 Colorimetric Assay Kit
| Code | Size | Price |
|---|
| MBL-4805 | 100 Assays | £420.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
dry ice
Storage:
-20°C. Please refer to datasheet for additional information
Documents
Further Information
Description:
Caspases are members of the cysteine aspartic acid-specific protease family,
which may be activated by a variety of signals including death receptor ligation,
DNA damage, serum starvation, stress, and many more. Active caspases
recognize a wide number of different substrates, leading to the activation of
several different apoptotic pathways. For example, CAD (caspase-activated
deoxyribonuclease), a protein involved in chromatin fragmentation into
nucleosome units, is indirectly activated by caspase-3, while ICAD (inhibitor of
caspase-activated deoxyribonuclease) is inactivated by caspase-3. The
cleavage of several structural proteins by caspases leads to the unique apoptosis
cell morphology of chromatin condensation, nucleus fragmentation and
cytoplasmic integrity. Detection of caspase activity is recognized as a standard
method to measure apoptosis activity. The most common method to measure
caspase activity is via the fluorometric or colorimetric detection of the cleavage
of caspase-specific substrates.
Gene IDs:
Human: 22861 Mouse: 12370
Kit Components:
Cell Lysis Buffer, 2X Reaction Buffer, 1M DTT, Substrate IETD-pNA (10mM), Inhibitor IETD-FMK (1mM), 100mMpNA (p-nitroanilide)
Shelf Life:
see label
Target:
Caspase-8
References
1). Berro AI, et al., J Immunol, 173, 2174 (2004)
2). Dinnen RD, et al., J Biol Chem, 282, 26675 (2007)
3). Evens AM, et al., Clin Cancer Res, 10, 1481 (2004)
4). Miyaji M, et al., J Exp Med, 202, 249 (2005)
5). Numata K, et al., J Immunol, 178, 3777 (2007)
6). Villani MG, et al, Cancer Res, 66, 3238 (2006)