IMMUNOCYTO CD107a Detection Kit
| Code | Size | Price |
|---|
| MBL-4844 | 50 Assays | £430.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
4°C
Storage:
4°C
Documents
Further Information
Description:
When T-cells and NK-cells recognize target cells, the contents of the lytic granules,
including proteins such as perforin, granzymes and granulysin, are secreted by
degranulation. CD107a, also known as lysosome-associated membrane protein 1
(LAMP-1), is a glycoprotein present in the membrane of cytotoxic granules. As a
result of degradation by these lytic molecules, CD107 is exposed on the cell surface.
Thus surface-exposed CD107 is a marker of secretion of the lytic granule contents, and
the detection of CD107 exposed on the cell surface enables rapid assessment of
cell-mediated cytotoxicity and detection of antigen specific cytotoxic T-cells.
The ?CD107a mobilization assay? is the most commonly used method for detection of
CD107a. In this method, the cells are cultured in the presence of CD107a monoclonal
antibody after antigen stimulation, which enables sensitive detection of CD107a. The
IMMUNOCYTO™ CD107a Detection kit is a reagent for detection of CD107a exposed
on the cell surface after antigen stimulation by CD107a mobilization assay.
Gene IDs:
Human: 3916 Mouse: 16783
Kit Components:
FITC labeled anti-human CD107a monoclonal antibody, FITC labeled mouse IgG1 isotype control, Monensin, Cell suspension solution
Shelf Life:
see label
Target:
CD107a
References
1). Rubio V. et al, Nat Med, 9: 1337-82 (2003)
2). Betts MR. et al, J Immunol methods, 281: 65-78 (2003)