Autophagy Ab Sampler Set

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MBL
Product Code: MBL-8485
Product Group: Cell Based Assays
Supplier: MBL
CodeSizePrice
MBL-8485Antibodies: 25 ul each, Positive control: 10 Tests£588.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Regulatory Status: RUO
Shipping:
4°C
Storage:
-20°C

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Further Information

Description:
Autophagy is a process of intracellular bulk degradation in which cytoplasmic components including organelles are sequestered within double-membrane vesicles. Autophagosomes deliver the contents to the lysosome/vacuole for degradation. This process aids in cell survival during starvation periods by eliminating nutrients being needed for unnecessary cellular processes. Recently, autophagy has been suggested to play an important role in development, differentiation, neurodegeneration, infection and cancer. MBLI provides a useful set of antibodies that can detect endogenous cellular factors for autophagy. The Autophagy Ab Sample Set includes popular autophagy targets for Beclin1, Atg16L, Atg5, p62 and LC3. The MBLI LC3 antibodies can clearly detect both LC3-I and LC3-II in western blotting. The MBLI Autophagy Antibody sample set is an economical approach to testing autophagy across multiple targets for many different applications.
Kit Components:
Anti-LC3 pAb (PM036Y), Anti-LC3 mAb (M186-3Y), Anti-LC3 mAb (M152-3Y), Anti-Beclin 1 pAb (PD017Y), Anti-Atg16L pAb (PM040Y), Anti-p62 (SQSTM1) pAb (PM045Y), Anti-Atg5 pAb (PM050Y), Positive control for anti-LC3 (PM036-PNY) *PM036-PNY: human LC3B overexpressed 293T 1x100000 cells were suspended with 100uL of Laemmli?s sample buffer. This product contains 5% 2-mercaptoethanol. Handle with care.
Shelf Life:
1 year
Target:
Autophagy

References

PM036 1) Hasui, K., et al., Acta Hitochem. Cytochem, 44, 119-131 (2011) [IHC-P] 2) Salazar, M., et al., Methods Enzymol. 489, 297-317 (2011) [WB, IC] 3) Bodemann, B. O., et al., Cell 144, 253-267 (2011) [IC] 4) Kobayashi, H., et al., Mol. Genet. Metab. 102, 170-175 (2011) [IHC-P] 5) Kaminskyy, V., et al., Autophagy 7, 83-90 (2011) [WB, IC, FCM] 6) Blanchet, F. P., et al., Immunity 32, 654-669 (2010) [WB] 7) Kimura, S., et al., Methods Enzymol. 452, 1-12 (2009) [WB] M152-3 1) Joubert, P. E., et al., J. Exp. Med. 209, 1029-1047 (2012) [IC] 2) Sims, J. J., et al., Nat. Methods 9, 303-309 (2012) [IC] 3) Ho, H., et al., J. Biol. Chem. 286, 12509-12523 (2011) [WB] 4) Saiki, S., et al., Autophagy 7, 176-187 (2011) [WB] 5) Eng, K. E., et al., Autophagy 6, 634-641 (2010) [FCM] 6) Wang, Y., et al., J. Biol. Chem. 283, 4766-4777 (2008) [WB, IC] PD017 1) Berliocchi, L., et al., Mol. Pain 7, 83 (2011) [WB] 2) Russo, R., et al., Cell Death Dis. 2, e144 (2011) [WB, IHC-Fr] 3) Matsunaga, K., et al., J.Cell Biol. 190, 511-521 (2010) [WB] PM040 1) Young, M. M., et al., J. Biol. Chem. 287, 12455-12468 (2012) [IC] 2) Takahashi, Y., et al., Autophagy 7, 61-73 (2011) [IC] 3) Matsunaga, K., et al., J.Cell Biol. 190, 511-521 (2010) [IC]PM036 1) Hasui, K., et al., Acta Hitochem. Cytochem, 44, 119-131 (2011) [IHC-P] 2) Salazar, M., et al., Methods Enzymol. 489, 297-317 (2011) [WB, IC] 3) Bodemann, B. O., et al., Cell 144, 253-267 (2011) [IC] 4) Kobayashi, H., et al., Mol. Genet. Metab. 102, 170-175 (2011) [IHC-P] 5) Kaminskyy, V., et al., Autophagy 7, 83-90 (2011) [WB, IC, FCM] 6) Blanchet, F. P., et al., Immunity 32, 654-669 (2010) [WB] 7) Kimura, S., et al., Methods Enzymol. 452, 1-12 (2009) [WB] M152-3 1) Joubert, P. E., et al., J. Exp. Med. 209, 1029-1047 (2012) [IC] 2) Sims, J. J., et al., Nat. Methods 9, 303-309 (2012) [IC] 3) Ho, H., et al., J. Biol. Chem. 286, 12509-12523 (2011) [WB] 4) Saiki, S., et al., Autophagy 7, 176-187 (2011) [WB] 5) Eng, K. E., et al., Autophagy 6, 634-641 (2010) [FCM] 6) Wang, Y., et al., J. Biol. Chem. 283, 4766-4777 (2008) [WB, IC] PD017 1) Berliocchi, L., et al., Mol. Pain 7, 83 (2011) [WB] 2) Russo, R., et al., Cell Death Dis. 2, e144 (2011) [WB, IHC-Fr] 3) Matsunaga, K., et al., J.Cell Biol. 190, 511-521 (2010) [WB] PM040 1) Young, M. M., et al., J. Biol. Chem. 287, 12455-12468 (2012) [IC] 2) Takahashi, Y., et al., Autophagy 7, 61-73 (2011) [IC] 3) Matsunaga, K., et al., J.Cell Biol. 190, 511-521 (2010) [IC]PM036 1) Hasui, K., et al., Acta Hitochem. Cytochem, 44, 119-131 (2011) [IHC-P] 2) Salazar, M., et al., Methods Enzymol. 489, 297-317 (2011) [WB, IC] 3) Bodemann, B. O., et al., Cell 144, 253-267 (2011) [IC] 4) Kobayashi, H., et al., Mol. Genet. Metab. 102, 170-175 (2011) [IHC-P] 5) Kaminskyy, V., et al., Autophagy 7, 83-90 (2011) [WB, IC, FCM] 6) Blanchet, F. P., et al., Immunity 32, 654-669 (2010) [WB] 7) Kimura, S., et al., Methods Enzymol. 452, 1-12 (2009) [WB] M152-3 1) Joubert, P. E., et al., J. Exp. Med. 209, 1029-1047 (2012) [IC] 2) Sims, J. J., et al., Nat. Methods 9, 303-309 (2012) [IC] 3) Ho, H., et al., J. Biol. Chem. 286, 12509-12523 (2011) [WB] 4) Saiki, S., et al., Autophagy 7, 176-187 (2011) [WB] 5) Eng, K. E., et al., Autophagy 6, 634-641 (2010) [FCM] 6) Wang, Y., et al., J. Biol. Chem. 283, 4766-4777 (2008) [WB, IC] PD017 1) Berliocchi, L., et al., Mol. Pain 7, 83 (2011) [WB] 2) Russo, R., et al., Cell Death Dis. 2, e144 (2011) [WB, IHC-Fr] 3) Matsunaga, K., et al., J.Cell Biol. 190, 511-521 (2010) [WB] PM040 1) Young, M. M., et al., J. Biol. Chem. 287, 12455-12468 (2012) [IC] 2) Takahashi, Y., et al., Autophagy 7, 61-73 (2011) [IC] 3) Matsunaga, K., et al., J.Cell Biol. 190, 511-521 (2010) [IC]PM036 1) Hasui, K., et al., Acta Hitochem. Cytochem, 44, 119-131 (2011) [IHC-P] 2) Salazar, M., et al., Methods Enzymol. 489, 297-317 (2011) [WB, IC] 3) Bodemann, B. O., et al., Cell 144, 253-267 (2011) [IC] 4) Kobayashi, H., et al., Mol. Genet. Metab. 102, 170-175 (2011) [IHC-P] 5) Kaminskyy, V., et al., Autophagy 7, 83-90 (2011) [WB, IC, FCM] 6) Blanchet, F. P., et al., Immunity 32, 654-669 (2010) [WB] 7) Kimura, S., et al., Methods Enzymol. 452, 1-12 (2009) [WB] M152-3 1) Joubert, P. E., et al., J. Exp. Med. 209, 1029-1047 (2012) [IC] 2) Sims, J. J., et al., Nat. Methods 9, 303-309 (2012) [IC] 3) Ho, H., et al., J. Biol. Chem. 286, 12509-12523 (2011) [WB] 4) Saiki, S., et al., Autophagy 7, 176-187 (2011) [WB] 5) Eng, K. E., et al., Autophagy 6, 634-641 (2010) [FCM] 6) Wang, Y., et al., J. Biol. Chem. 283, 4766-4777 (2008) [WB, IC] PD017 1) Berliocchi, L., et al., Mol. Pain 7, 83 (2011) [WB] 2) Russo, R., et al., Cell Death Dis. 2, e144 (2011) [WB, IHC-Fr] 3) Matsunaga, K., et al., J.Cell Biol. 190, 511-521 (2010) [WB] PM040 1) Young, M. M., et al., J. Biol. Chem. 287, 12455-12468 (2012) [IC] 2) Takahashi, Y., et al., Autophagy 7, 61-73 (2011) [IC] 3) Matsunaga, K., et al., J.Cell Biol. 190, 511-521 (2010) [IC]PM045 1) Seto, S., et al., Cell Microbiol. 14, 710-727 (2012) [IC] 2) Huang, Y., et al., Autophagy 7, 1132-1144 (2011) [WB] 3) Itakura, E. and Mizushima N., J.Cell Biol. 192, 17-27 (2011) [WB] 4) Dupont, N., et al., Cell Host Microbe 6, 137-149 (2009) [IC] 5) Waguri, S., et al., Methods Enzymol. 453, 181-196 (2009) [WB, IHC-Fr] PM050 1) Myeku, N., et al., J. Biol. Chem. 286, 22426-22440 (2011) [WB]