Anti-HA-tag pAb

MBL
Product Code: MBL-561-5
Product Group: Primary Antibodies
Supplier: MBL
CodeSizePrice
MBL-561-5500 ul£599.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunocytochemistry (ICC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
4°C
Storage:
-20°C

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Further Information

Applications:
WB - 1:1,000 IP - 1 uL/sample ICC - 1:200
Background:
Epitope tagging has widely been accepted technique that fuse an epitope peptide to a certain protein as a marker for gene expression. With this technique, the gene expression can be easily monitored on western blotting, immunoprecipitation and immunofluorescence utilizing with an antibody that recognizes such an epitope. Amino acid sequences that are widely used for the epitope tagging are as follow; YPYDVPDYA (HA-tag), EQKLISEEDL (Myc-tag) and YTDIEMNRLGK (VSV-G-tag), which corresponding to the partial peptide of Influenza hemagglutinin protein, Human c-myc gene product and Vesicular stomatitis virus glycoprotein respectively.
Formulation:
PBS/50% glycerol, pH 7.2
Immunogen Translated:
YPYDVPDYA (HA-tag)-KLH
Reactivity:
This antibody reacts with HA-tag on Western blotting.
Shelf Life:
1 year
Source:
This antibody was purified from rabbit serum using protein A agarose. The rabbit was immunized with KLH conjugated synthetic peptide, KLH-YPYDVPDYA (HA-tag).
Target:
HA

References

1) Fukunaka, A., et al., J. Biol. Chem. 286, 16363-16373 (2011) 2) Mimura, Y., et al., J. Cell Sci. 123, 2014-2024 (2010) 3) Murano, K., et al., Mol. Cell Biol. 28, 3114-3126 (2008) 4) Shirai, A., et al., J. Biol. Chem. 283, 10745-10752 (2008) 5) Sun, P., et al., Genes Cells 11, 1097-1113 (2006) 6) Arao, Y., et al., Mol. Endocrinol 18, 2255-2267 (2004) 7) Myojin, R., et al., Biol. Reprod 71, 926-932 (2004) 8) Zhang, A., et al., J. Biol. Chem. 279, 33799-33805 (2004) 9) Murayama, Y., et al., J. Cell Sci. 117, 3379-3388 (2004) 10) Saito, T. T., et al., Nucleic Acids Res. 32, 3325-3339 (2004) 11) Toji, S., et al., Genes Cells 9, 383-397 (2004) 12) Yasuda, S., et al., Endocrinology 145, 1730-1738 (2004) 13) Sekiguchi, T., et al., J. Biol. Chem. 279, 8343-8350 (2004) 14) Abe, T., et al., J. Cell Sci. 116, 155-168 (2003) 15) Sun, P., et al., J. Biol. Chem. 278, 4063-4071 (2003) 16) Furusawa, M., et al., J. Biol. Chem. 277, 50885-50892 (2002) 17) Katsu, R., et al., J. Biol. Chem. 277, 44220-44228 (2002) 18) Zama, T., et al., J. Biol. Chem. 277, 23909-23918 (2002) 19) Nishizawa, H., et al., J. Biol. Chem. 277, 1586-1592 (2002) 20) Kamimoto, T., et al., J. Biol. Chem. 276, 37520-37528 (2001) 21) Kojima, T., et al., J. Biol. Chem. 276, 32247-32256 (2001) 22) Sekiguchi, T., et al., J. Biol. Chem. 276, 7246-7257 (2001) 23) Izumi, M., et al., Nucleic Acid Res. 28, 4769-4777 (2000) 24) Matsuda, S., et al., J. Biol. Chem. 274, 34515-34518 (1999) 25) Takahashi, M., et al., J. Virol. 73, 7812-7816 (1999) 26) Koizumi, J., et al., J. Biol. Chem. 274, 11125-11131 (1999) 27) Kondo, T., et al., Mol. Cell Biol. 19, 1136-1143 (1999) 28) Furuse, M., et al., J. Cell Biol. 143, 391-401 (1998) 29) Shimomura, T., et al., Mol. Cell Biol. 18, 5485-5491 (1998)