CycLex Cyclic GMP dependent protein kinase (cGK) Assay Kit

Other - 1) Monitoring the purification of cGK. 2) Screening inhibitors or activators of cGK. 3) Detecting the effects of pharmacological agents on cGK.

Nitric oxide (NO) and a broad spectrum of hormones, drugs, and toxins raise intracellular cGMP concentrations and thereby regulate a great variety of functions, including smooth muscle relaxation, neuronal excitability, and epithelial electrolyte transport. Pfeifer et al. (1996) noted that depending on the tissue, an increase in cGMP concentration leads to the activation of different receptors, such as cyclic nucleotide phosphodiesterases. The identification of the physiologic mediator of cGMP is complicated by the existence of 2 forms of cGMP-dependent protein kinase (cGK), types I and II, which are encoded by distinct genes. Smooth muscle, platelets, and cerebellum contain high concentrations of cGK-I, whereas cGK-II is highly concentrated in brain, lung, and intestinal mucosa. The function of cGK-II is not well understood, although there is evidence that it mediates intestinal secretion of water and electrolytes induced by the E. coli toxin STa and the intestinal peptide guanylin. Activation of cyclic GMP-dependent protein kinase (cGK) is an important event in the regulation of blood pressure and platelet function. Upstream signals include the generation of nitric oxide (NO) by NO synthases and the subsequent rise in cGMP levels mediated by NO-dependent guanyl cyclases (GCs). The identification of new cGK activators by high throughput screening (HTS) may lead to the development of a novel class of therapeutics for the treatment of cardiovascular diseases.

The CycLex Research Product CycLex®Cyclic GMP dependent protein kinase (cGK) Assay kit is primarily designed to measure the activities of purified the cGK family of kinases for the rapid and sensitive evaluation of inhibitors or activators. The phospho-specific monoclonal antibody used in this assay kit has been demonstrated to recognize the phospho-threonine 68/119 residues on G-kinase substrate, which is phosphorylated by cGK family members. Additionally, column fractions of any cultured primary cell, cell line, or tissue homogenate can be assayed for cGK family activity with the CycLex Research Product cGK Assay kit if the appropriate dose of cGK specific inhibitor, e.g. (Rp)-8-pCPT-cGMPS.

Human: 5592 Mouse: 19091

Microplate (coated with recombinant G-kinase substrate), 10?~Wash Buffer, Kinase Buffer, 20?~ATP, HRP conjugated Detection Antibody, Substrate Reagent, Stop Solution

cGKs