Anti-HA-tag mAb
| Code | Size | Price |
|---|
| MBL-M180-3 | 200 ul | £269.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG2b κ
Antibody Clonality: Monoclonal
Antibody Clone: TANA2
Regulatory Status: RUO
Applications:
- Flow Cytometry
- Immunocytochemistry (ICC)
- Immunoprecipitation (IP)
- Western Blot (WB)
Shipping:
4°C
Storage:
-20°C
Documents
Further Information
Applications:
WB - 0.1 ug/mL (chemiluminescence detection system) IP - 2 ug/300 uL of cell extract from 3x106 cells FCM - 1 ug/mL (final concentration) ICC - 1 ug/mL
Background:
Epitope tag technique has widely been accepted technique that fuses an epitope peptide to a certain protein as a marker for gene expression. With this technique, the gene expression can be easily monitored on western blotting, immunoprecipitation, and immunofluorescence, utilizing with an antibody that recognizes such an epitope. HA-tag (YPYDVPDYA) sequence is widely used for the epitope tag, which corresponding to the partial peptide of Influenza hemagglutinin protein.
Concentration:
1 mg/mL
Formulation:
PBS containing 50% Glycerol (pH 7.2). No preservative is contained.
Immunogen Translated:
Synthetic peptide binding KLH, YPYDVPDYA (HA tag)
Reactivity:
This antibody reacts with N-terminal and C-terminal HA-tagged proteins.
Shelf Life:
1 year
Source:
Purified IgG from hybridoma supernatant
Target:
HA
References
1) Toyonaga, K., et al., PLoS One. 9, e88747 (2014)
2) Iguchi, M., et al., J. Biol. Chem. 288, 22019-22032 (2013) [WB]