7-AAD solution
Code | Size | Price |
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MBL-FP00020050 | 500 tests | £187.00 |
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Overview
Regulatory Status: RUO
Application: Flow Cytometry
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Further Information
Background:
The 7-AAD (7-amino-actinomycin D) solution can be used to exclude non-viable cells in flow cytometry assays. It is excited by 488 laser light and is detected by the far red 650nm long-pass filter. Unlike propidium iodide, 7-AAD has minimal spectral overlap with the fluorescence emissions of PE and FITC.
References
Kubo, R. T., et al., J Immunol. 142, 2736-2742 (1989) Gascoigne, N. R., J. Biol. Chem. 265, 9296-9301 (1990) Kruisbeek, A. M., et al., Curr Protoc Immunol. l, 3-12 (2004) Saint-Ruf, C., et al., Nature. 406, 524-527 (2000)