NAD+/NADH Colorimetric Assay Kit
| Code | Size | Price |
|---|
| E-BC-K804-M-96T | 96T | £378.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
Ice packs
Storage:
-20°C
Further Information
Assay Time:
70 min
Detection Instrument:
Microplate reader
Detection Range:
0.02-5.0 umol/L
Detection Type:
Colorimetric method
Inter cv:
9.1
Intra cv:
1.8
Result Type:
Quantitative
Sensitivity:
0.02 umol/L
Target Synonym:
NAD+/NADH
Test Principle:
Detect total content of NAD+ and NADH
Ethanol generates acetaldehyde under the action of enzyme. Meanwhile, NAD+ is reduced to NADH, NADH, under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. Therefore, the total content of NAD+ and NADH can be quantified by measure the OD value at 450 nm.
Detect NADH
After treating sample, heat at 60oC water bath for 30 min. the NAD+ of the sample is decomposed and only NADH remains. NADH reduces WST-8 to form yellow product, and the amount of NADH is determined by measure the OD value at 450 nm.
Detect NAD+ and NAD+/NADH
The content of NAD+ and the ratio of NAD+/NADH in the sample can be obtained according to the total content of NAD+ and NADH obtained of the first two steps as well as the separate content of NADPH.
Note: NADP+ and NADPH have no effect on the determination results.
Tested Sample Types:
animal tissue, cell