Glutaminase (GLS) Activity Assay Kit

Elabscience
Product Code: E-BC-K660-M
Supplier: Elabscience
CodeSizePrice
E-BC-K660-M-96T96T£291.00
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Overview

Regulatory Status: RUO
Shipping:
Ice packs
Storage:
-20°C

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Further Information

Assay Time:
80min
Detection Instrument:
Microplate reader
Detection Range:
0.003-18.0 U/L
Detection Type:
Colorimetric method
Inter cv:
8.3
Intra cv:
5
Result Type:
Enzyme Activity
Sensitivity:
0.003 U/L
Target Synonym:
GLS
Test Principle:
Glutamine is decomposed to produce glutamic acid under the action of glutaminase. Glutamic acid is further transformed by glutamic acid dehydrogenase. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of glutaminase can be calculated by measuring the change of absorbance value at 450 nm.
Tested Sample Types:
serum (plasma), animal, plant tissue