Glutamine Colorimetric Assay Kit
| Code | Size | Price |
|---|
| E-BC-K853-M-48T | 48T | £274.00 |
Quantity:
| E-BC-K853-M-96T | 96T | £371.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
Ice packs
Storage:
-20°C
Further Information
Assay Time:
70 min
Detection Instrument:
Microplate reader
Detection Range:
0.036-2.0 mmol/L
Detection Type:
Colorimetric method
Inter cv:
4.7
Intra cv:
3.2
Result Type:
Quantitative
Sensitivity:
0.036 mmol/L
Target Synonym:
Gln
Test Principle:
Gln is hydrolyzed to produce glutamic acid under the action of glutaminase. Glutamic acid is further catalyzed by glutamic acid dehydrogenase. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The content of Gln can be calculated by measuring the change of absorbance value at 450 nm.
Tested Sample Types:
serum (plasma), animal, plant tissue