BCA Protein Colorimetric Assay Kit
| Code | Size | Price |
|---|
| E-BC-K318-M-96T | 96T | £143.00 |
Quantity:
| E-BC-K318-M-500Assays | 500Assays | £177.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Application: Quantification
Shipping:
Ice packs
Storage:
RT
Further Information
Assay Time:
40 min
Detection Instrument:
Microplate reader
Detection Range:
0.0165-1 mg/mL
Detection Type:
Colorimetric method
Inter cv:
4.5
Intra cv:
2.2
Result Type:
Quantitative
Sensitivity:
0.0165 mg/mL
Target Synonym:
TP
Test Principle:
Cu2+ can be reduced to Cu+ by protein in alkaline condition. Cu+ can combine with BCA reagent and form purple complex, which has a maximum absorption peak at 562 nm. The absorbance value is proportional to the protein concentration. Therefore, the protein concentration can be calculated according to the OD value.
Tested Sample Types:
Serum,plasma,cell culture supernatant,tissue,cells
References
- https://www.cell.com/immunity/pdf/S1074-7613(23)00025-0.pdf
- https://www.sciencedirect.com/science/article/pii/S1385894723005685
- https://www.sciencedirect.com/science/article/pii/S0048969722071790
- https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-022-01646-9
- https://www.sciencedirect.com/science/article/pii/S2590006421000971
- https://www.sciencedirect.com/science/article/pii/S0889159121005067
- https://pubs.acs.org/doi/abs/10.1021/acs.jmedchem.1c02025
- https://www.mdpi.com/2013838
- https://www.sciencedirect.com/science/article/pii/S0147651322005929
- https://www.mdpi.com/1836124
- https://www.sciencedirect.com/science/article/pii/S0925443923000273
- https://www.hindawi.com/journals/omcl/2021/9966663/