Protein Carbonyl Colorimetric Assay Kit (Tissue And Serum Samples)
| Code | Size | Price |
|---|
| E-BC-K117-S-50Assays | 50Assays | £197.00 |
Quantity:
| E-BC-K117-S-100Assays | 100Assays | £251.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Application: Quantification
Shipping:
Ice packs
Storage:
2-8°C
Further Information
Assay Time:
240 min
Detection Instrument:
Spectrophotometer
Detection Range:
0.02-10 nmol/mgprot
Detection Type:
Colorimetric method
Inter cv:
8.6
Intra cv:
4.5
Result Type:
Quantitative
Sensitivity:
0.02 nmol/mgprot
Test Principle:
The content of protein carbonyl increased after oxidation, and the carbonyl group reacted with 2, 4-dinitrophenylhydrazine to form a reddish brown precipitate. The absorbance can be measured at 370 nm after the precipitation is dissolved. The carbonyl content can be calculated indirectly.
Tested Sample Types:
Serum,plasma,hydrothorax,cell culture supernatant,tissue
References
- https://www.sciencedirect.com/science/article/pii/S2213231721003414
- https://www.mdpi.com/1988290
- https://www.mdpi.com/1420170
- https://www.sciencedirect.com/science/article/pii/S0147651319307377
- https://academic.oup.com/fqs/advance-article-abstract/doi/10.1093/fqsafe/fyac032/6582386
- https://www.sciencedirect.com/science/article/pii/S0024320520303325
- https://onlinelibrary.wiley.com/doi/abs/10.1002/jez.2603