Non-esterified Free Fatty Acids (NEFA) Colorimetric Assay Kit
| Code | Size | Price |
|---|
| E-BC-K013-M-48T | 48T | £177.00 |
Quantity:
| E-BC-K013-M-96T | 96T | £224.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Application: Quantification
Shipping:
Ice packs
Storage:
2-8°C
Further Information
Assay Time:
30 min
Detection Instrument:
Microplate reader
Detection Range:
0.15-1.5 mmol/L
Detection Type:
Colorimetric method
Inter cv:
5.1
Intra cv:
3.3
Result Type:
Quantitative
Sensitivity:
0.15 mmol/L
Target Synonym:
NEFA
Test Principle:
Under the condition of weak acidity, non-esterified free fatty acids (NEFA) react with nantokite to form copper soap, which has a specific absorption peak at 715nm. The content of NEFA can be calculated indirectly by measuring the OD value at 715 nm.
Tested Sample Types:
Animal tissue
References
- https://www.sciencedirect.com/science/article/pii/S0142961222005427
- https://www.sciencedirect.com/science/article/pii/S0048969722056844
- https://www.sciencedirect.com/science/article/pii/S004896972105405X
- https://onlinelibrary.wiley.com/doi/abs/10.1111/pai.13651
- https://www.mdpi.com/2072-6643/14/3/440
- https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8200454/
- https://www.sciencedirect.com/science/article/pii/S0753332220303309
- https://www.sciencedirect.com/science/article/pii/S2405844020319769
- https://link.springer.com/article/10.1007/s10787-018-0519-4
- https://www.jstage.jst.go.jp/article/jos/71/5/71_ess21389/_article/-char/ja/