Free Cholesterol (FC) Colorimetric Assay Kit
| Code | Size | Price |
|---|
| E-BC-K004-M-48T | 48T | £157.00 |
Quantity:
| E-BC-K004-M-96T | 96T | £177.00 |
Quantity:
| E-BC-K004-M-500Assays | 500Assays | £344.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Application: Quantification
Shipping:
Ice packs
Storage:
2-8°C
Further Information
Assay Time:
20 min
Detection Instrument:
Microplate reader
Detection Range:
0.07-24 mmol/L
Detection Type:
Colorimetric method
Inter cv:
5.2
Intra cv:
1.9
Result Type:
Quantitative
Sensitivity:
0.07 mmol/L
Target Synonym:
FC
Test Principle:
Free cholesterol produces 4-cholestenone and hydrogen peroxide under the oxidation of cholesterol oxidase. In the presence of 4-aminoamylpyridine and phenol, peroxidase catalyze hydrogen peroxide to form red quinone compounds of benzoquinone imine phenizone. The color depth of the generated quinones is directly proportional to the cholesterol content.
Tested Sample Types:
Serum, plasma, tissue
References
- https://www.sciencedirect.com/science/article/pii/S0002944022002425
- https://www.sciencedirect.com/science/article/pii/S0377840121001942
- https://www.tandfonline.com/doi/abs/10.2147/VHRM.S396295