Total Iron Colorimetric Assay Kit
| Code | Size | Price |
|---|
| E-BC-K772-M-48T | 48T | £177.00 |
Quantity:
| E-BC-K772-M-96T | 96T | £224.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Application: Quantification
Shipping:
Ice packs
Storage:
2-8°C
Further Information
Assay Time:
80min
Detection Instrument:
Microplate reader
Detection Range:
0.4-50 umol/L
Detection Type:
Colorimetric method
Inter cv:
1.5
Intra cv:
1.3
Result Type:
Quantitative
Sensitivity:
0.4 umol/L
Test Principle:
Under the action of reductant, iron ions in samples can be reduced into ferrous ions (Fe2+). The latter then bind to probe and form complexes, which has a maximum absorption peak at 593 nm. The concentration of iron can be calculated by measuring the OD value at 593 nm indirectly.
Tested Sample Types:
serum, tissue
References
- https://www.sciencedirect.com/science/article/pii/S0944711322004093
- https://onlinelibrary.wiley.com/doi/abs/10.1002/tox.23740
- https://academic.oup.com/biolreprod/article-abstract/104/6/1347/6161978
- https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8075096/