Sucrose Colorimetric Assay Kit
| Code | Size | Price |
|---|
| E-BC-K161-S-50Assays | 50Assays | £197.00 |
Quantity:
| E-BC-K161-S-100Assays | 100Assays | £251.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Application: Quantification
Shipping:
Ice packs
Storage:
2-8°C
Further Information
Assay Time:
40 min
Detection Instrument:
Spectrophotometer
Detection Range:
0.32-70 umol/mL
Detection Type:
Colorimetric method
Inter cv:
8.5
Intra cv:
3.4
Result Type:
Quantitative
Sensitivity:
0.32 umol/mL
Test Principle:
Sucrose in plant tissue is hydrolyzed to glucose and fructose in boiling water bath under acidic conditions. 5-hydroxymethyl furfural was synthesized from fructose under acid condition and measure the ultraviolet absorption of 5-hydroxymethyl furfural. Glucose must be dissimilated into ketose structure and reduced to obtain 5-hydroxymethylfurfural, but the rate of isomerization of glucose to ketose is very slow. Therefore, the ultraviolet absorption of glucose is much smaller than fructose.
Tested Sample Types:
Plant tissue