Glycogen Colorimetric Assay Kit (Liver/Muscle Samples)
| Code | Size | Price |
|---|
| E-BC-K073-S-50Assays | 50Assays | £177.00 |
Quantity:
| E-BC-K073-S-100Assays | 100Assays | £210.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Application: Quantification
Shipping:
Ice packs
Storage:
2-8°C
Further Information
Assay Time:
50 min
Detection Instrument:
Spectrophotometer
Detection Range:
1.80-180 mg/g liver
Detection Type:
Colorimetric method
Inter cv:
7.3
Intra cv:
3.7
Result Type:
Quantitative
Sensitivity:
1.80 mg/g liver tiss
Test Principle:
Under the presence of concentrated sulfuric acid, glycogen can be dehydrated to furfural derivatives. Furfural derivatives can form blue compound with anthracenone. The concentration of the compound can be measured by colorimetric quantification at 620 nm with glucose standard buffer of same treatment. Glycogen is quite stable in concentrated alkali solution. Heating the tissue sample in concentrated alkali solution before color development will remove other components and keep the glycogen.
Tested Sample Types:
Animal liver,muscle
References
- https://www.mdpi.com/1836124
- https://academic.oup.com/endo/advance-article-abstract/doi/10.1210/endocr/bqac217/6967064