Ethanol Colorimetric Assay Kit
| Code | Size | Price |
|---|
| E-BC-K891-M-48T | 48T | £251.00 |
Quantity:
| E-BC-K891-M-96T | 96T | £331.00 |
Quantity:
| E-BC-K891-M-500Assays | 500Assays | £813.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Application: Quantification
Shipping:
Ice packs
Storage:
-20°C
Further Information
Assay Time:
30 min
Detection Instrument:
Microplate reader
Detection Range:
0.27-10.0 umol/mL
Detection Type:
Colorimetric method
Inter cv:
5
Intra cv:
3.5
Result Type:
Quantitative
Sensitivity:
0.27 umol/mL
Test Principle:
Ethanol dehydrogenase can catalyze oxidative dehydrogenation of ethanol to acetaldehyde, and NAD+ is reduced to produce NADH. NADH, under the action of 1-mPMS, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. Therefore, ethanol content can be quantified by measure the OD value at 450 nm.
Tested Sample Types:
serum, plasma, wine