Total Cholesterol and Cholesteryl Ester Fluorometric Assay Kit
| Code | Size | Price |
|---|
| E-BC-F032-48T | 48T | £264.00 |
Quantity:
| E-BC-F032-96T | 96T | £344.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Application: Quantification
Shipping:
Ice packs
Storage:
-20°C
Further Information
Assay Time:
20 min
Detection Instrument:
Fluorescence Microplate Reader
Detection Range:
0.12-30 umol/L
Detection Type:
Fluorescence method
Inter cv:
7.3
Intra cv:
1.7
Result Type:
Quantitative
Sensitivity:
0.12 umol/L
Test Principle:
Total Cholesterol (TC) includes free cholesterol (FC) and cholesteryl esters (CE). Cholesterol ester can be hydrolyzed by cholesterol esterase to produce cholesterol and free fatty acid. Cholesterol is oxidized by cholesterol oxidase to produce δ4-cholestenone and hydrogen peroxide. In the presence of the enzyme and probe, hydrogen peroxide can be catalyzed to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 590 nm is proportional to the cholesterol concentration.
Tested Sample Types:
Serum, plasma, animal tissue, cells
References
- https://www.mdpi.com/1908036