Free Fatty Acids (FFA) Fluorometric Assay Kit
| Code | Size | Price |
|---|
| E-BC-F039-48T | 48T | £277.00 |
Quantity:
| E-BC-F039-96T | 96T | £378.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Application: Quantification
Shipping:
Ice packs
Storage:
-20°C
Further Information
Assay Time:
70 min
Detection Instrument:
Fluorescence Microplate Reader
Detection Range:
0.58-20 umol/L
Detection Type:
Fluorescence method
Inter cv:
5.3
Intra cv:
4.2
Result Type:
Quantitative
Sensitivity:
0.58 umol/L
Target Synonym:
FFA;NEFA
Test Principle:
Free fatty acids produce acyl coenzyme A in the presence of acyl synthase, which produces hydrogen Free fatty acids produce acyl coenzyme A in the presence of acyl synthase, which produces hydrogen peroxide in the presence of acyl oxidase. In the presence of the enzyme and probe, hydrogen peroxide react to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 590 nm is directly proportional to the concentration of free fatty acids.
Tested Sample Types:
Serum, plasma, animal tissue, cells
References
- https://www.nature.com/articles/s41467-022-35481-1
- https://onlinelibrary.wiley.com/doi/abs/10.1111/cpr.13332
- https://link.springer.com/article/10.1186/s13098-022-00921-x
- https://www.sciencedirect.com/science/article/pii/S0378874122009011
- https://www.sciencedirect.com/science/article/pii/S0955286319301330
- https://link.springer.com/article/10.1007/s11356-021-14871-w
- https://www.tandfonline.com/doi/abs/10.1080/01480545.2020.1784190