Total Superoxide Dismutase (T-SOD) Activity Assay Kit (Hydroxylamine Method)

Elabscience
Product Code: E-BC-K019-M
Supplier: Elabscience
CodeSizePrice
E-BC-K019-M-48T48T£210.00
Quantity:
E-BC-K019-M-96T96T£264.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Regulatory Status: RUO
Application: Activity Assay
Shipping:
Ice packs
Storage:
Reagent4:-20°C;Others:2-8°C

Images

1 / 2
2 / 2

Documents

Further Information

Assay Time:
70 min
Detection Instrument:
Microplate reader
Detection Range:
2.4-61 U/mL
Detection Type:
Colorimetric method
Inter cv:
5.6
Intra cv:
5.5
Result Type:
Enzyme Activity
Sensitivity:
2.4 U/mL
Target Synonym:
T-SOD
Test Principle:
The superoxide anion free radical (O2-) can be produced by xanthine and xanthine oxidase reaction system, O2- oxidize hydroxylamine to form nitrite, it turn to purple under the reaction of developer. When the measured samples containing SOD, the SOD can specifically inhibit superoxide anion free radical (O2-). The inhibitory effect of SOD can reduce the formation of nitrite, the absorbance value of sample tube is lower than control tube. Calculate the SOD of sample according to the computational formula.
Tested Sample Types:
Serum,plasma,urine,cells,cell culture supernatant,tissue

References

  • https://www.sciencedirect.com/science/article/pii/S2452199X23000154
  • https://www.sciencedirect.com/science/article/pii/S0304389421020707
  • https://cmbl.biomedcentral.com/articles/10.1186/s11658-023-00416-1
  • https://www.mdpi.com/1970288
  • https://www.mdpi.com/2076-3921/11/10/1879
  • https://www.mdpi.com/1905716
  • https://onlinelibrary.wiley.com/doi/abs/10.1111/pce.14263
  • https://www.hindawi.com/journals/omcl/2022/2232365/
  • https://www.mdpi.com/article/10.3390/ijms232315400
  • https://www.nature.com/articles/s41419-020-02777-3
  • https://www.sciencedirect.com/science/article/pii/S0147651322000240
  • https://www.mdpi.com/1422-0067/23/21/13531