Ascorbate Peroxidase (APX) Activity Assay Kit
| Code | Size | Price |
|---|
| E-BC-K353-S-50Assays | 50Assays | £224.00 |
Quantity:
| E-BC-K353-S-100Assays | 100Assays | £291.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Application: Activity Assay
Shipping:
Ice packs
Storage:
2-8°C
Further Information
Assay Time:
60 min
Detection Instrument:
Spectrophotometer
Detection Range:
0.071-47 U/g tissue
Detection Type:
Colorimetric method
Inter cv:
6.4
Intra cv:
4.8
Result Type:
Enzyme Activity
Sensitivity:
0.071 U/g tissue
Target Synonym:
APX
Test Principle:
Ascorbate Peroxidase (APX) can catalyze the reaction between ascorbic acid (ASA) and hydrogen peroxide (H2O2), and ASA can be oxidized to monodehydroascorbic acid (MDASA). The absorbance of solution at 290 nm will decline as the oxidation of ASA. The APX activity can be calculated by detecting the decrease of A290.
Tested Sample Types:
Plant tissue
References
- https://www.sciencedirect.com/science/article/pii/S1674205221001659
- https://onlinelibrary.wiley.com/doi/abs/10.1111/jpi.12504
- https://www.frontiersin.org/articles/10.3389/fpls.2022.941388/full
- https://www.sciencedirect.com/science/article/pii/S0981942820300784
- https://www.mdpi.com/2223-7747/10/3/541
- https://peerj.com/articles/11888/
- https://journals.ashs.org/hortsci/view/journals/hortsci/56/9/article-p1066.xml