Aspartate Aminotransferase (AST/GOT) Activity Assay Kit (Reitman-Frankel Method)

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Elabscience
Product Code: E-BC-K236-M
Supplier: Elabscience
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E-BC-K236-M-48T48T£157.00
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E-BC-K236-M-96T96T£197.00
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E-BC-K236-M-500Assays500Assays£398.00
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Overview

Regulatory Status: RUO
Application: Activity Assay
Shipping:
Ice packs
Storage:
2-8°C

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Further Information

Assay Time:
80 min
Detection Instrument:
Microplate reader
Detection Range:
1.1-72.3 IU/L
Detection Type:
Colorimetric method
Inter cv:
6.8
Intra cv:
5.3
Result Type:
Enzyme Activity
Sensitivity:
1.1 IU/L
Target Synonym:
AST;GOT
Test Principle:
AST/GOT enables alpha-ketoglutaric acid and aspartic acid to displace amino and keto groups to form glutamic acid and oxaloacetic acid. Oxaloacetic acid can decarboxylate itself to form Pyroracemic acid during the reaction. Pyroracemic acid reacted with 2,4-dinitrophenylhydrazine(DNPH) to form 2,4, dinitrophenylhydrazone showing reddish brown in alkaline solution. Measure the OD values and calculate the enzyme activity.
Tested Sample Types:
Serum,plasma,animal tissue,cells,cell culture supernatant

References

  • https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.202204909
  • https://www.nature.com/articles/s41467-022-29722-6
  • https://aasldpubs.onlinelibrary.wiley.com/doi/abs/10.1002/hep.29573
  • https://www.sciencedirect.com/science/article/pii/S2162253122002165
  • https://www.sciencedirect.com/science/article/pii/S2211124722011457
  • https://pubs.acs.org/doi/abs/10.1021/acsami.1c12235
  • https://www.sciencedirect.com/science/article/pii/S0304389420328673
  • https://pubs.acs.org/doi/abs/10.1021/acs.est.2c00516
  • https://www.sciencedirect.com/science/article/pii/S1742706120307509
  • https://www.sciencedirect.com/science/article/pii/S1742706121007108
  • https://www.sciencedirect.com/science/article/pii/S2352345X22001928
  • https://onlinelibrary.wiley.com/doi/abs/10.1111/cpr.13332