Alanine Aminotransferase (ALT/GPT) Activity Assay Kit (Reitman-Frankel Method)
| Code | Size | Price |
|---|
| E-BC-K235-M-48T | 48T | £157.00 |
Quantity:
| E-BC-K235-M-96T | 96T | £197.00 |
Quantity:
| E-BC-K235-M-500Assays | 500Assays | £398.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Application: Activity Assay
Shipping:
Ice packs
Storage:
2-8°C
Further Information
Assay Time:
80 min
Detection Instrument:
Microplate reader
Detection Range:
0.75-72.3 IU/L
Detection Type:
Colorimetric method
Inter cv:
9
Intra cv:
5.3
Result Type:
Enzyme Activity
Sensitivity:
0.75 IU/L
Target Synonym:
ALT;GPT
Test Principle:
ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid at pH 7.4 and 37°C. Then phenylhydrazine was added to form phenylhydrazone with pyruvic acid. Phenylhydrazone is reddish brown under alkaline conditions. ALT activity can be calculated by measuring the OD values at 510 nm.
Tested Sample Types:
Serum,plasma,animal tissue,cells,cell culture supernatant
References
- https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.202204909
- https://www.nature.com/articles/s41467-022-35481-1
- https://aasldpubs.onlinelibrary.wiley.com/doi/abs/10.1002/hep.29573
- https://www.sciencedirect.com/science/article/pii/S2162253122002165
- https://www.sciencedirect.com/science/article/pii/S2211124722011457
- https://pubs.acs.org/doi/abs/10.1021/acsami.1c12235
- https://pubs.acs.org/doi/abs/10.1021/acs.est.2c00516
- https://www.sciencedirect.com/science/article/pii/S0304389420328673
- https://www.sciencedirect.com/science/article/pii/S2352345X22001928
- https://www.sciencedirect.com/science/article/pii/S1742706121007108
- https://www.sciencedirect.com/science/article/pii/S1043661819308473
- https://www.sciencedirect.com/science/article/pii/S0048969722030819