Lactate Dehydrogenase (LDH) Cytotoxicity Colorimetric Assay Kit

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Elabscience
Product Code: E-BC-K771-M
Product Group: Biochemical Assay Kits
Supplier: Elabscience
CodeSizePrice
E-BC-K771-M-96T96T£170.00
Quantity:
E-BC-K771-M-500Assays500Assays£291.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Regulatory Status: RUO
Application: Activity Assay
Shipping:
Ice packs
Storage:
-20°C

Documents

Further Information

Assay Time:
30 min
Detection Instrument:
Microplate reader
Detection Type:
Colorimetric method
Result Type:
Enzyme Activity
Target Synonym:
LDH
Test Principle:
Lactate dehydrogenase catalyzes the reaction of lactic acid with NAD+ to produce pyruvic acid and NADH. NADH, under the action of PMS, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. Therefore, LDH activity can be quantified by measure the OD value at 450 nm.
Tested Sample Types:
cells

References

  • https://www.nature.com/articles/s42003-020-01511-1
  • https://www.sciencedirect.com/science/article/pii/S0009279720310875
  • https://www.sciencedirect.com/science/article/pii/S1567576922004969
  • https://www.sciencedirect.com/science/article/pii/S0006291X18303528
  • https://www.sciencedirect.com/science/article/pii/S0026286223000237
  • https://link.springer.com/article/10.1007/s11033-023-08256-0
  • https://www.sciencedirect.com/science/article/pii/S221475001930157X
  • https://www.tandfonline.com/doi/abs/10.1080/11101849.2022.2094070
  • https://www.sciencedirect.com/science/article/pii/S2405580822001480