Acid Phosphatase (ACP) Activity Assay Kit
| Code | Size | Price |
|---|
| E-BC-K010-M-96T | 96T | £143.00 |
Quantity:
| E-BC-K010-M-500Assays | 500Assays | £251.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Application: Activity Assay
Shipping:
Ice packs
Storage:
-20°C
Further Information
Assay Time:
30 min
Detection Instrument:
Microplate reader
Detection Range:
0.2-50 U/L
Detection Type:
Colorimetric method
Inter cv:
4.5
Intra cv:
2.8
Result Type:
Enzyme Activity
Sensitivity:
0.2 U/L
Target Synonym:
ACP
Test Principle:
Disodium p-nitrobenzene phosphate (p-NPP), a widely used phosphatase chromogenic substrate, can form p- nitrophenol under the action of acid phosphatase. Under alkaline conditions, p-nitrophenol is yellow and has a maximum absorption peak at 405 nm. The darker of the yellow product is, the higher of the ACP activity is. Therefore, the activity of ACP can be calculated by measuring the OD value at 405 nm.
Tested Sample Types:
Serum, plasma, tissue