Mouse IFNα(Interferon Alpha) CLIA Kit
| Code | Size | Price |
|---|
| E-CL-M0716-24T | 24T | £223.00 |
Quantity:
| E-CL-M0716-48T | 48T | £350.00 |
Quantity:
| E-CL-M0716-96T | 96T | £442.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Regulatory Status: RUO
Application: Chemiluminescent Immunoassay (CLIA)
Shipping:
Ice packs
Storage:
An unopened kit can be stored at 2-8°C for 1 month. If the kit is not supposed to be used within 1 month store the items separately according to the storage table in the manual once the kit is received.
Documents
Further Information
Abbreviation:
IFNα
Assay Time:
3.5h
Cross reactivity:
This kit recognizes Mouse IFNα in samples.No significant cross-reactivity or interference between Mouse IFNα and analogues was observed
Detection Range:
15.63-1000 pg/mL
Detection Type:
Chemiluminescence
Precision:
Both intra-CV and inter-CV are < 10%.
Recovery rate:
80%-120%
Sensitivity:
9.38 pg/mL
Target Species:
Mouse
Test Principle:
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IFNα. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IFNα and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IFNα, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse IFNα. You can calculate the concentration of Mouse IFNα in the samples by comparing the RLU value of the samples to the standard curve.
Tested Sample Types:
serum, plasma and other biological fluids
Type:
Sandwich
UNIProt ID:
P01572,P01573
Usage:
This ELISA kit applies to the in vitro quantitative determination of Mouse IFNα concentrations in serum, plasma and other biological fluids.