L-Lactic Acid/Lactate (LA) Colorimetric Assay Kit
| Code | Size | Price |
|---|
| E-BC-K044-M-48T | 48T | £224.00 |
Quantity:
| E-BC-K044-M-96T | 96T | £277.00 |
Quantity:
| E-BC-K044-M-500Assays | 500Assays | £679.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
Ice packs
Storage:
2-8°C
Further Information
Assay Time:
30 min
Detection Instrument:
Microplate reader
Detection Range:
0.12-7.0 mmol/L
Detection Type:
Colorimetric method
Inter cv:
3.5
Intra cv:
1.4
Result Type:
Quantitative
Sensitivity:
0.10 mmol/L
Test Principle:
Using NAD+ as H+ receptor, LDH catalyzes the reaction of lactic acid and NAD+ to generate pyruvic acid and NADH respectively. NBT is reduced to a kind of purple compound during the reaction. Measure the OD value at 530 nm, and the concentration of lactic acid can be calculated.
Tested Sample Types:
Serum,plasma,cell culture supernatant,tissue,cells
References
- https://www.sciencedirect.com/science/article/pii/S0092867422015379
- https://www.nature.com/articles/s41423-022-00894-x
- https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8419046/
- https://jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-022-02521-y
- https://www.sciencedirect.com/science/article/pii/S0223523423000818
- https://www.sciencedirect.com/science/article/pii/S2352304222002331
- https://link.springer.com/article/10.1186/s12964-022-00882-8
- https://link.springer.com/article/10.1186/s12935-022-02845-y
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9207367/
- https://www.mdpi.com/1400748
- https://www.sciencedirect.com/science/article/pii/S258900422101138X
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8408126/