CuZn/Mn Superoxide Dismutase (CuZn-SOD/Mn-SOD) Activity Assay Kit (Hydroxylamine Method)
| Code | Size | Price |
|---|
| E-BC-K022-M-48T | 48T | £210.00 |
Quantity:
| E-BC-K022-M-96T | 96T | £264.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
Ice packs
Storage:
Reagent4:-20°C;Others:2-8°C
Further Information
Assay Time:
70 min
Detection Instrument:
Microplate reader
Detection Range:
1.35-62 U/mL
Detection Type:
Colorimetric method
Inter cv:
9.6
Intra cv:
5.1
Result Type:
Enzyme Activity
Sensitivity:
1.35 U/mL
Target Synonym:
CuZn-SOD;Mn-SOD;T-SOD
Test Principle:
Superoxide anion (O2-) produced by xanthine and xanthine oxidase system can oxidize hydroxylamine to form nitrite which appear purplish red after chromogenic reaction. The SOD in the sample has a specific inhibitory effect on superoxide anion (O2-), can reduce the content of nitrite. The OD value is lower than control and the activity of SOD is calculated through the formula. There are two kinds of SOD (CuZn-SOD, Mn-SOD) in the cells of higher animals, and the sum of them is equal to the total SOD. The activity of Mn-SOD in the sample will lost after sample pretreatment, but the activity of CuZn-SOD will not.
Tested Sample Types:
Serum,plasma,urine,cells,cell culture supernatant,tissue
References
- https://onlinelibrary.wiley.com/doi/abs/10.1111/pce.14263
- https://onlinelibrary.wiley.com/doi/abs/10.1111/pce.14263
- https://link.springer.com/article/10.1007/s00204-022-03431-x
- https://www.mdpi.com/1926090
- https://www.hindawi.com/journals/omcl/2022/7655142/
- https://www.sciencedirect.com/science/article/pii/S0024320522001382
- https://www.hindawi.com/journals/omcl/2020/8365056/
- https://www.mdpi.com/1861292
- https://www.sciencedirect.com/science/article/pii/S1001074222000717
- https://aasldpubs.onlinelibrary.wiley.com/doi/abs/10.1002/lt.26421
- https://www.mdpi.com/1409316
- https://europepmc.org/article/pmc/pmc9500298?javascript_support=no