Glucose Uptake Fluorometric Assay Kit
| Code | Size | Price |
|---|
| E-BC-F041-96T | 96T | £599.00 |
Quantity:
| E-BC-F041-500Assays | 500Assays | £1,415.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
Ice packs
Storage:
-20°C
Further Information
Assay Time:
100 min
Detection Instrument:
Fluorescence microplate reader
Detection Range:
0.02-0.3 nmol/uL
Detection Type:
Fluorescence method
Result Type:
Quantitative
Sensitivity:
0.02 nmol/uL
Test Principle:
2-DG is up-taken by the cells, converted to 2-DG-6P, which is catalyzed by glucose dehydrogenase to produce 6PDG. Meanwhile, NADP+ is converted to NADPH. The generated NADPH converts the probe into fluorescent substances under the action of myocardial yellow transferase. The glucose uptake can be calculated by measuring the fluorescence intensity at the excitation wavelength of 530 nm and the emission wavelength of 590 nm.
Tested Sample Types:
cells
References
- https://www.sciencedirect.com/science/article/pii/S1043661822003930
- https://onlinelibrary.wiley.com/doi/abs/10.1002/hed.27229
- https://www.mdpi.com/1433254