Sucrose Fluorometric Assay Kit
| Code | Size | Price |
|---|
| E-BC-F042-96T | 96T | £311.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
Ice packs
Storage:
-20°C
Further Information
Assay Time:
70 min
Detection Instrument:
Fluorescence microplate reader
Detection Range:
0.15-15 umol/L
Detection Type:
Fluorescence method
Inter cv:
6.5
Intra cv:
2.3
Result Type:
Quantitative
Sensitivity:
0.15 umol/L
Test Principle:
Sucrose can be hydrolyzed by sucrase to produce glucose under acidic conditions, which is catalyzed by glucose oxidase to produce hydrogen peroxide. In the presence of HRP (horse radish peroxidase), hydrogen peroxide reacts with the fluorescent probe to form red fluorescent substance. The sucrose content can be calculated by measuring the fluorescence intensity at the excitation wavelength of 535 nm and the emission wavelength of 590 nm.
Tested Sample Types:
Plant tissue