Non-esterified Free Fatty Acids (NEFA) Colorimetric Assay Kit

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Elabscience
Product Code: E-BC-K014
Product Group: Biochemical Assay Kits
Supplier: Elabscience
CodeSizePrice
E-BC-K014-96T96T£422.00
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Overview

Regulatory Status: RUO
Shipping:
Ice packs
Storage:
2-8°C

Documents

Further Information

Assay Time:
30 min
Detection Instrument:
Microplate reader, Biochemistry analyzer
Detection Range:
0.01-3.0 mmol/L
Detection Type:
Colorimetric method
Result Type:
Quantitative
Target Synonym:
NEFA
Test Principle:
NEFA and can react with coenzyme A and form acetyl-CoA under the catalysis of acetyl-CoA-synthetase (ACS). Acetyl-CoA can produce H2O2 when catalyzed by acetyl-CoA-oxidase (ACOD). Then H2O2 react with TOOS and 4-amino-antipyrine (4-APP) to generate a colored substrate under the catalysis of peroxidase (POD). The colored substrate has a maximum absorption peak at 546 nm. Measure the OD value at 546 nm and calculate the NEFA content indirectly.
Tested Sample Types:
Serum,plasma,tissue homogenate,cells,cell supernatant

References

  • https://www.sciencedirect.com/science/article/pii/S1074761322006033
  • https://www.nature.com/articles/s41467-022-33493-5
  • https://www.nature.com/articles/s41467-022-28641-w
  • https://link.springer.com/article/10.1186/s12902-022-01143-y
  • https://www.mdpi.com/1574186