α-Ketoglutarate Dehydrogenase(α-KGDH)Activity Assay Kit
| Code | Size | Price |
|---|
| E-BC-K083-M-96T | 96T | £317.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
Ice packs
Storage:
-20°C
Further Information
Assay Time:
40 min
Detection Instrument:
Microplate reader
Detection Range:
8.3-42.3 U/L
Detection Type:
Colorimetric method
Inter cv:
8.5
Intra cv:
1.5
Result Type:
Enzyme Activity
Sensitivity:
8.3 U/L
Target Synonym:
α-KGDH
Test Principle:
α-ketoglutarate dehydrogenase (α-KGDH) is a key enzyme in the tricarboxylic acid (TCA) cycle. While the substrate being catalyzed by α-KGDH, NAD+ is reduced to NADH, and electrons are transfered to WST-8 under the action of hydrogen transmitter to produce yellow product. The activity of α-KGDH can be calculated by measuring the change of absorbance value at 450 nm
Tested Sample Types:
serum, plasma, animal and plant tissue